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ADAM12 peptide (Aminoterminal end) (ab41204)

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Overview

  • Product nameADAM12 peptide (Aminoterminal end)See all ADAM12 proteins and peptides ...
  • Protein descriptionThis peptide was used as an immunogen for ab39155 - ADAM12 antibody - Aminoterminal end.

    • Properties

  • Purity> 95 % by SDS-PAGE
  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.001% Tween 20, 30mM HEPES, 2mM EDTA, 150mM Sodium chloride, pH 6.75
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab41204 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB
  • Application notesWB: Use at an assay dependent dilution.
    This peptide can be used with studies using ab39155. Dilution optimised using Chromogenic detection.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        A disintegrin and metalloproteinase domain 12ADA12_HUMANADAM 12
        ADAM metallopeptidase domain 12ADAM12Disintegrin and metalloproteinase domain-containing protein 12MCMPMCMPMltnaMeltrin alphaMeltrin-alphaMLTNMLTNAOTTHUMP00000046766
      see all
  • FunctionInvolved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors.
  • Tissue specificityIsoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. Not detected in brain, lung, liver, kidney or pancreas.
  • Sequence similaritiesContains 1 disintegrin domain.
    Contains 1 EGF-like domain.
    Contains 1 peptidase M12B domain.
  • DomainThe cysteine-rich domain supports cell adhesion through syndecans and triggers signaling events that lead to beta-1 integrin-dependent cell spreading. In carcinomas cells the binding of this domain to syndecans does not allow the integrin-mediated cell spreading.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications    The precursor is cleaved by a furin endopeptidase.
  • Cellular localizationSecreted and Cell membrane.

    • Target information above from: UniProt accession O43184 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for ADAM12 peptide (Aminoterminal end) (ab41204)

    ab41204 has not yet been referenced specifically in any publications.

    Publishing research using ab41204 ? Please let us know so that we can cite the reference in this datasheet

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    I am using peptide ab41204 as a blocking peptide to blockab39155. The western blot produces lots of non specific bands, although I do see a band that looks about the correct size of the short form. The data is similar to data produced using 2 separate ant...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"