Anti-dimethyl Arginine antibody [21C7] - ChIP Grade (ab413)
Overview
- Product nameAnti-dimethyl Arginine antibody [21C7] - ChIP Grade
- DescriptionMouse monoclonal [21C7] to dimethyl Arginine - ChIP Grade
- SpecificityThis antibody reacts with free and bound asymmetric NG-NG-dimethyl arginine. It is not known whether the antibody reacts with symmetric dimethyl arginine.
- Tested applicationsIP, WB, ChIP more details
- Immunogen
Asymmetric NG -N G -dimethyl arginine (two methyl groups on one of the terminal nitrogen atoms of the guanidinium group)
- General notes
This antibody will be of use in chromatin and transcription factor research to monitor methylation status. Since it is specific to dimethyl arginine it can be used in parallel with ab412 (detects monomethyl and dimethyl arginine) and ab414 (detects monomethyl arginine only) to monitor the exact modification status.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferConstituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose
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Concentration information loading... - PurityProtein A purified
- Primary antibody notes This antibody will be of use in chromatin and transcription factor research to monitor methylation status. Since it is specific to dimethyl arginine it can be used in parallel with ab412 (detects monomethyl and dimethyl arginine) and ab414 (detects monomethyl arginine only) to monitor the exact modification status.
- Clonality Monoclonal
- Clone number21C7
- IsotypeIgM
- Light chain typekappa
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab413 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IP | IP: Use at an assay dependent dilution. |
| WB | WB: 1/50 - 1/500. |
| ChIP | ChIP: Use at an assay dependent concentration. Use 5µl for 4x106 cells. |
Target
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Alternative names
- Di methyl Arginine antibody
References for Anti-dimethyl Arginine antibody [21C7] - ChIP Grade (ab413)
This product has been referenced in:
- Souki SK et al. Arginine methylation of the ICP27 RGG box regulates ICP27 export and is required for efficient herpes simplex virus 1 replication. J Virol 83:5309-20 (2009). WB . Read more (PubMed: 19321610) »
- Dolzhanskaya N et al. Alternative splicing modulates protein arginine methyltransferase-dependent methylation of fragile X syndrome mental retardation protein. Biochemistry 45:10385-93 (2006). Read more (PubMed: 16922515) »
