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Anti-Dengue Virus E glycoprotein antibody [DE1] (ab41349)

Submit an Abreview Q&A (7)Specific References (2)

Overview

  • Product nameAnti-Dengue Virus E glycoprotein antibody [DE1]See all Dengue Virus E glycoprotein primary antibodies ...
  • Description
    Mouse monoclonal [DE1] to Dengue Virus E glycoprotein
  • SpecificityThis antibody reacts with a conformational epitope on the envelope gylcoprotein of Dengue Virus 1, 2, 3 and 4.
  • Tested applicationsICC/IF, ELISA, Dot Blot more details
  • Species reactivity
    Reacts with: Dengue Virus 1, Dengue Virus 2, Dengue Virus 3, Dengue Virus 4
  • Immunogen

    Full length native protein purified from Dengue Virus 2 (16681) infected supernatant

Properties

Applications

Our Abpromise guarantee covers the use of ab41349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF ICC/IF: 1/5 - 1/20.
ELISA ELISA: Use at an assay dependent dilution. This antibody has been used in ELISA for the detection of degue IgM in patient serum samples.
Dot Blot Dot: Use at an assay dependent dilution.
  • Application notesIs unsuitable for or WB.

    • Target

    • RelevanceDengue virus E glycoprotein is thought to be essential for membrane fusion and is also involved in binding to cellular receptors. It is the primary antigen responsible for inducing the protective immune response.
    • Database links
      • Alternative names
          Dengue glycoprotein E antibodyDengue virus glycoprotein E antibody

      Anti-Dengue Virus E glycoprotein antibody [DE1] images

      • Immunocytochemistry/ Immunofluorescence - Dengue Virus envelope glycoprotein E antibody [DE1] (ab41349)
        Immunocytochemistry/ Immunofluorescence - Dengue Virus envelope glycoprotein E antibody [DE1] (ab41349)
        Dengue Virus 2 infected Vero cells stained with ab41349 antibody against Dengue Virus envelope glycoprotein E (green). Cell nuclei are blue.
      • Immunocytochemistry/ Immunofluorescence - Dengue Virus E glycoprotein antibody [DE1] (ab41349)
        Immunocytochemistry/ Immunofluorescence - Dengue Virus E glycoprotein antibody [DE1] (ab41349)
        Dengue Virus 2 infected Vero cells stained according to protocol with ab41349 antibody against Dengue Virus envelope glycoprotein E (green). Cell nuclei are blue.

      References for Anti-Dengue Virus E glycoprotein antibody [DE1] (ab41349)

      This product has been referenced in:
      • Cardosa MJ  et al. A nitrocellulose membrane based IgM capture enzyme immunoassay for etiological diagnosis of dengue virus infections. Clin Diagn Virol 3:343-50 (1995). Read more (PubMed: 15566815) »
      • Cardosa MJ  et al. IgM capture ELISA for detection of IgM antibodies to dengue virus: comparison of 2 formats using hemagglutinins and cell culture derived antigens. Southeast Asian J Trop Med Public Health 23:726-9 (1992). Read more (PubMed: 1298081) »

      See all 2 Publications for this product

      Publishing research using ab41349 ? Please let us know so that we can cite the reference in this datasheet

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      Submit a Question for ab41349

      Displaying 1 - 7 of 7 results for Q&A

      10
      Votes: Highest First

      I have some equiry about two antibodies, DE1 (ab41349) and DE2 (ab41696). Since, your technical specialist had replied me before that "we have tested them on recombinant DIII coated plates and they were not reactive." I would like to know what is the reco...

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      Thank you for your enquiry. Basically, the epitopes for DE1 and DE2 are complex, conformational epitopes and probably non-linear - and since the immunogen is not a peptide, the amino acid sequence is not known. The information on the recombinant D...

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      Inquiry: please let me know that the Anti-Dengue Virus E glycoprotein antibody [DE1] (ab41349) will bind to domain III of all four dengue serotypes or not as it is mentioned that it will react with conformational epitopes on envelope but is it on domain I...

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      Unfortunately, we do not know where the epitope for DE1 is on the E glycoprotein. However, we do know that it does not bind recombinant DIII from the pET expression system. This therefore suggests that this antibody will not recognise endogenous domain II...

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      Inquiry: Hi, I would like to buy your: ab41349 dengue antibody. Would it be possible to know which secondary antibody is likely to go with it please? It would be good if it is conjugated to FITC. This is to be used for IF. Best wishes

      Thank you for contacting us.

      We searched on our web page and there are three products that I could recommend to you. They are all three against the IgG2b of mouse and were used for ICC/IF. The species the three products were raised in is differe...

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      Today I received the ordered product with reference ab41349. The solution containing the antibody is reddish and I was wondering if that is normal.

      Also, I would like to know the mAb concentration in the product. If it is not possible, it wo...

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      Thank you for contacting us.

      ab41349 is a tissue culture supernatant therefore it is normal for it to be reddish as this may be due to the colour of the media. It is likely that there are additional reagents and/or proteins that might obliterate ...

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      Thanks for your reply.

      The antibody that you mentioned is react only with dengue type 2 and not with other serotypes.



      warm regards,

      Thank you for your response.

      Currently, we have antibodies against Dengue virus which detect Type 1 and Type 3 separately.

      If you need any further assistance, please do not hesitate to contact me.

      Dear Sir/ Madam,

      please let me know why anti dengue envelope antibody (code: ab41349 and ab 41696) are not suitable for WB as mentioned in datasheet. I am looking for anti dengue envelope antibody generated in mouse which is suitable for WB, EL...

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      Thank you for your enquiry and your interest in our products.

      I can confirm that the Western blot results with this antibody (ab41349: Anti-Dengue Virus E glycoprotein antibody [DE1]) have been inconclusive, thus we do not list WB as a tested ap...

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      One of our customers want to buy the antibody of Dengue Virus E glycoprotein. He would like to ask more specifically about the domain group of the antibody #ab41696 and #ab41349. It is because he will choose between these two regarding the domain group. ...

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      Thank you for your enquiry. DE1 (ab41349) and DE2 (ab41696) are monoclonal antibodies from two different fusion experiments but the immunizing protocol is the same for both fusions. As mentioned in the datasheets, although they are both dengue group s...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"