Anti-Noradrenaline transporter antibody (ab41559)
- Product nameAnti-Noradrenaline transporter antibodySee all Noradrenaline transporter primary antibodies ...
- DescriptionRabbit polyclonal to Noradrenaline transporter
- SpecificityAb41559 does not cross react with dopamine and serotonin transporters.
- Tested applicationsELISA, Dot Blot, WB, ICC/IF more details
- Species reactivityReacts with: Mouse, Rat, Human
The noradrenaline transporter antibodies were generated against unique N-terminal peptides that are unique to the noradrenaline transporter protein. The antibodies are raised utilizing linear, multiple antigenic peptides or cyclic peptide methodology.
- Positive controlRat brain cortical membranes.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.2% Sodium Azide
Constituents: PBS, BSA, HEPES
- Concentration information loading...
- PurityImmunogen affinity purified
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab41559 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot Blot||Dot: 1/4000.|
|WB||WB: 1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 70 kDa).|
|ICC/IF||ICC/IF: Use a concentration of 5 µg/ml.|
- RelevanceCatecholamine, a term used for the hormone adrenaline and its sequentially hydroxylated form noradrenaline, is involved in fight or flight responses. Noradrenaline is released from the post ganglionic sympathetic nerve endings and exerts its effects locally in the immediate vicinity of its release. In the CNS, noradrenaline is involved in a number of physiological responses including mood, sleep regulation, alertness and arousal, both cognitive and non-cognitive expression of behaviors, and control of the endocrine and autonomic nervous systems. Peripherally, noradrenaline is present in sympathetic nerve endings and has full control of the sympathetic nervous system. Noradrenaline released from pre-synaptic nerve terminals is reabsorbed (70-90%) by noradrenaline transporters and its biological effects are terminated. The noradrenaline transport via noradrenaline transporters is an active, Na+/Cl- dependent transport process mediated by noradrenaline transporters. Noradrenaline transporters constitute the primary mechanism for inactivation of synaptically released noradrenaline, are targets for multiple antidepressants and psychostimulants, and are deficient in affective and autonomic disorders. In rat brain, noradrenaline transporter is expressed in noradrenergic neuronal somata, axons and dendrites, and hippocampus and cortex, but is absent from epinephrine- and dopamine-containing neurons. At least 13 genetic variations have been reported in the noradrenaline transporter protein that affect noradrenaline re-uptake and concentrations in cerebrospinal fluid in humans. The association between these genetic variations in noradrenaline transporters and several psychiatric and cardiovascular disorders is just emerging. Recently, a single amino acid mutation (hNET-A457P) showed deficiency in noradrenaline transport in an orthostatic intolerance patient. Noradrenaline transporter protein consists of 617 amino acids and has 12 trans-membrane domains, a characteristic feature of many membrane associated solute transporters.
- Cellular localizationCell Membrane; multi-pass membrane protein
- NAT1 antibodyNET antibodyNET antibody
- NET1 antibodyNeurotransmitter transporter antibodyNorepinephrine transporter antibodySLC6A2 antibodySLC6A5 antibodySLC6A5 antibodySodium dependent noradrenaline transporter antibodySolute carrier family 6 (neurotransmitter transporter norepinephrine) member 5 antibodySolute carrier family 6 (neurotransmitter transporter, noradrenalin), member 2 antibodySolute carrier family 6 member 2 antibodySolute carrier family 6 member 2 antibodySolute carrier family 6 member 5 antibody
Anti-Noradrenaline transporter antibody images
Anti-Noradrenaline transporter antibody (ab41559) at 1/250 dilution + rat brain cortical membrane cell lysate at 70 µg
Predicted band size : 70 kDa
Observed band size : 80 kDa (why is the actual band size different from the predicted?)
Additional bands at : 28 kDa,32 kDa. We are unsure as to the identity of these extra bands.
ICC/IF image of ab41559 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41559, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Noradrenaline transporter antibody (ab41559)
ab41559 has not yet been referenced specifically in any publications.