Anti-TARDBP antibody (ab41972)

Overview

• Product nameAnti-TARDBP antibodySee all TARDBP primary antibodies ...
• Description
  Rabbit polyclonal to TARDBP
• Tested applicationsIHC-Fr, ICC/IF, WB more details
• Species reactivity
  Reacts with: Rat, Human
  Predicted to work with: Mouse, Chicken, Cow
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human TARDBP.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab41971.)

• Positive controlThis antibody gave a positive signal in the following human lysates: HeLa Whole Cell, HeLa Nuclear, Jurkat Whole Cell, Jurkat Nuclear, HepG2 Whole Cell, HepG2 Nuclear, A431 Whole Cell and K562 Nuclear - tumor cell line.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Microbiology
  • Organism
  • Virus
  • RNA Virus
  • ssRNA positive strand virus
  • HIV

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • RNA Processing
  • Splicing

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Other

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Amyotrophic lateral sclerosis

Applications

Target

• FunctionDNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR.
• Tissue specificityUbiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen.
• Involvement in diseaseDefects in TARDBP are the cause of amyotrophic lateral sclerosis type 10 (ALS10) [MIM:612069]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of ALS is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases.
• Sequence similaritiesContains 2 RRM (RNA recognition motif) domains.
• DomainThe RRM domains can bind to both DNA and RNA.
• Post-translational
  modificationsHyperphosphorylated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
  Ubiquitinated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
  Cleaved to generate C-terminal fragments in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
• Cellular localizationNucleus. In patients with frontotemporal lobar degeneration and amyotrophic lateral sclerosis, it is absent from the nucleus of affected neurons but it is the primary component of cytoplasmic ubiquitin-positive inclusion bodies.

Target information above from: UniProt accession Q13148 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 419453 Chicken
  • Entrez Gene: 23435 Human
  • Entrez Gene: 230908 Mouse
  • Entrez Gene: 298648 Rat
  • Omim: 605078 Human
  • SwissProt: Q5ZLN5 Chicken
  • SwissProt: Q13148 Human
  • SwissProt: Q921F2 Mouse

  • Unigene: 300624 Human
  • Unigene: 635053 Human
  • Unigene: 22453 Mouse
  • Unigene: 2633 Rat

  see all

• Alternative names
  ALS10 antibodyOTTHUMP00000002171 antibodyOTTHUMP00000002172 antibody

  OTTHUMP00000002173 antibodyTADBP_HUMAN antibodyTAR DNA binding protein 43 antibodyTAR DNA binding protein antibodyTAR DNA-binding protein 43 antibodyTARDBP antibodyTDP 43 antibodyTDP-43 antibodyTDP43 antibody

  see all

Anti-TARDBP antibody images

• 

  Western blot - TARDBP antibody (ab41972)
  All lanes : Anti-TARDBP antibody (ab41972) at 1/250 dilution
  
  Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
  Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
  Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844)
  Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
  Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate (ab14660)
  Lane 7 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 8 : K562 (Human erythromyeloblastoid leukemia cell line) Nuclear Lysate - tumor cell line (ab29309)
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 45 kDa
  Observed band size : 47 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 26 kDa,28 kDa. We are unsure as to the identity of these extra bands.
• 

  Immunocytochemistry/ Immunofluorescence - TARDBP antibody (ab41972)
  ICC/IF image of ab41972 stained human Hek293 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab41972, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).