Anti-MCAK antibody (ab42676)

Overview

• Product nameAnti-MCAK antibodySee all MCAK primary antibodies ...
• Description
  Rabbit polyclonal to MCAK
• Tested applicationsIHC-P, ICC/IF, WB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Cow, Chinese Hamster
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 650 to the C-terminus of Human MCAK.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab42675.)

• Positive controlThis antibody gave a signal in the following whole cell lysates; HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line) HEK293 (Human embryonic kidney cell line)

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Motor Proteins
  • Kinesin

Applications

Target

• FunctionPromotes ATP-dependent removal of tubulin dimers from microtubules. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis.
• Tissue specificityExpressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary; expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas. Isoform 2 is testis-specific.
• Sequence similaritiesBelongs to the kinesin-like protein family. MCAK/KIF2 subfamily.
  Contains 1 kinesin-motor domain.
• Developmental stageIsoform 2 is expressed in fetal testis.
• DomainThe microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.
• Post-translational
  modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation by STK12, regulates association with centromeres and kinetochores and the microtubule depolymerization activity.
• Cellular localizationCytoplasm > cytoskeleton. Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1 (By similarity). Centromeric localization requires the presence of BUB1 and SGOL2.

Target information above from: UniProt accession Q99661 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 11004 Human
  • Entrez Gene: 11004 Human
  • Entrez Gene: 73804 Mouse
  • Entrez Gene: 171529 Rat
  • Omim: 604538 Human
  • SwissProt: Q99661 Human
  • SwissProt: Q99661 Human
  • SwissProt: Q922S8 Mouse

  • SwissProt: Q62909 Rat
  • Unigene: 720061 Human
  • Unigene: 247651 Mouse
  • Unigene: 219966 Rat

  see all

• Alternative names
  4930402F02Rik antibodyESTM5 antibodyKIF 2C antibody

  kif2c antibodyKIF2C_HUMAN antibodyKinesein Family Member 2C antibodyKinesin family member 2C antibodykinesin like 6 (mitotic centromere associated kinesin) antibodyKinesin like 6 antibodyKinesin like protein 6 antibodyKinesin like protein KIF2C antibodyKinesin-like protein 6 antibodyKinesin-like protein KIF2C antibodyKNS L6 antibodyKNSL 6 antibodyKNSL6 antibodyMCAK antibodyMGC11883 antibodyMitotic centromere associated kinesin antibodyMitotic centromere-associated kinesin antibodyOTTHUMP00000010066 antibodyX83316 antibody

  see all

Anti-MCAK antibody images

• 

  Western blot - MCAK antibody (ab42676)
  All lanes : Anti-MCAK antibody (ab42676) at 1 µg/ml
  
  Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
  Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
  Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 81 kDa
  Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunocytochemistry/ Immunofluorescence - MCAK antibody (ab42676)
  ICC/IF image of ab42676 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab42676, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCAK antibody (ab42676)
  IHC image of MCAK staining in human liver carcinoma FFPE section, performed on a Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab42676, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.