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Overview

  • Product nameSHP1 peptideSee all SHP1 proteins and peptides ...
  • Protein descriptionSynthetic peptide corresponding to amino acids around serine 591 of human SHP1, the serine is phosphorylated.

    • Properties

  • Biological activityThe ab42772 peptide blocks antibodies that recognise amino acids around phosphorylated serine 591 of human SHP1. This sequence is highly conserved in rat and mouse SHP1.
  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab42772 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Blocking
    ELISA
  • Application notesBL: Use at a concentration of 1 µg/ml. Recommended for blocking antibody reactivity in Western Blot and Immunocytochemistry.
    ELISA: 10-100ng/well, established in 96-well Nunc immunoplates where peptide was bound to plates for 2hrs in 0.1M sodium carbonate buffer, pH 8.5.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        70 kda SHP1L protein70Z-SHPEC 3.1.3.48
        HCPHCPHHematopoietic cell phosphataseHematopoietic cell protein tyrosine phosphataseHematopoietic cell protein-tyrosine phosphataseHPTP1CProtein tyrosine phosphatase 1CProtein tyrosine phosphatase non receptor type 6Protein tyrosine phosphatase SHP1Protein-tyrosine phosphatase 1CProtein-tyrosine phosphatase SHP-1PTN6_HUMANPTP-1CPTP1CPTPN6SH PTP 1SH-PTP1SHP 1LSHP1SHP1LTyrosine-protein phosphatase non-receptor type 6
      see all
  • FunctionPlays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation.
  • Tissue specificityIsoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
  • Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
    Contains 2 SH2 domains.
    Contains 1 tyrosine-protein phosphatase domain.
  • Post-translational
    modifications    Phosphorylated on serine and tyrosine residues.
  • Cellular localizationCytoplasm. Nucleus. In neurons, translocates into the nucleus after treatment with angiotensin II.

    • Target information above from: UniProt accession P29350 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    SHP1 peptide images

    • Western blot - SHP1 peptide (ab42772)
      Western blot - SHP1 peptide (ab42772)
      All lanes : Anti-SHP1 (phospho S591) antibody (ab41436)

      Lane 3 : human Jurkat cells treated with pervanadate (1 mM) for 30 min
      Lane 4 : human Jurkat cells treated with pervanadate (1 mM ( for 30 min) and exposed to lambda phosphatase
      Lane 5 : human Jurkat cells treated with pervanadate (1 mM) for 30 min with phospho-SHP1 (Ser-591) peptide
      Lane 6 : human Jurkat cells treated with pervanadate (1 mM) for 30 min with non-specific phospho-serine peptide

    References for SHP1 peptide (ab42772)

    ab42772 has not yet been referenced specifically in any publications.

    Publishing research using ab42772 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"