Overview
Product nameSARS Nucleocapsid Protein proteinSee all SARS Nucleocapsid Protein proteins and peptides ...
Protein descriptionRecombinant fragment corresponding to amino acids 340/390 of SARS Nucleocapsid Protein. Contains immunodominant regions.
Expression hostE. coli
Properties
Purity> 95
% by SDS-PAGE
Purification notesSepharose derived purification.
Biological activityImmunoreactive with sera of SARS infected individuals.
FormLiquid
Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: None
Constituents: 50% Glycerol, 60mM Sodium chloride, 50mM Tris HCl
Concentration information loading...
SequenceL DDKDPQFKDN VILLNKHIDA YKTFPPTEPK KDKKKKTDEA QPLPQRQKKQ
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab43030
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|
Application
|
Notes |
| ELISA |
|
| WB |
|
Application notesELISA: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.
Excellent antigen for detection of SARS with minimal specificity problems.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
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Alternative names
Nucleocapsid proteinNucleoproteinProtein NSARS coronavirus N proteinSARS CoVSARSCoVSevere acute respiratory syndrome
see all
RelevanceSevere Acute Respiratory Syndrome (SARS), an emerging disease characterized by atypical pneumonia, has recently been attributed to a novel coronavirus (SARS-CoV). SARS is caused by a human coronavirus, which are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46 kDa nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics. The nucleocapsid protein of SARS shares little homology with nucleocapsid proteins of other members of the coronavirus family. Nucleocapsid proteins of other coronavirus have been reported to be involved in forming the viral core and also in the packaging and transcription of the viral RNA.
Cellular localizationInside the virion, complexed with the viral RNA.
May be associated with cellular membranes where it participates in viral RNA synthesis and virus budding.
References for SARS Nucleocapsid Protein protein (ab43030)
ab43030
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
