Anti-Anterior Gradient 2 antibody (ab43043)
Overview
- Product nameAnti-Anterior Gradient 2 antibodySee all Anterior Gradient 2 primary antibodies ...
- DescriptionRabbit polyclonal to Anterior Gradient 2
- Tested applicationsWB, ICC/IF, IHC-P more details
- Species reactivityReacts with: Human
- Immunogen
Recombinant fusion protein (Human)
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: Tris -
Concentration information loading... - PurityIgG fraction
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab43043 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 2 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa). |
| ICC/IF | ICC/IF: Use a concentration of 4 - 8 µg/ml. |
| IHC-P | IHC-P: Use at an assay dependent concentration. |
Target
- FunctionRequired for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth.
- Tissue specificityExpressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid gland and in estrogen receptor (ER)-positive breast cancer cell lines.
- Sequence similaritiesBelongs to the AGR family.
- Cellular localizationSecreted. Endoplasmic reticulum.
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Database links
- Entrez Gene: 10551 Human
- Omim: 606358 Human
- SwissProt: O95994 Human
- Unigene: 530009 Human
Target information above from: UniProt accession
O95994
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- AG 2 antibodyAG 2 protein antibodyAG-2 antibody
- AG2 antibodyAG2 protein antibodyAGR 2 antibodyAGR2 antibodyAGR2_HUMAN antibodyAnterior gradient 2 homolog (Xenopus laevis) antibodyanterior gradient 2 homolog antibodyAnterior gradient homolog 2 antibodyAnterior gradient protein 2 homolog antibodyGOB 4 antibodyGOB4 antibodyHAG 2 antibodyhAG-2 antibodyhAG2 antibodyHAG2/R antibodyHPC 8 antibodyHPC8 antibodyPDIA17 antibodyProtein disulfide isomerase family A member 17 antibodysecreted cement gland homolog antibodySecreted cement gland protein XAG 2 homolog antibodySecreted cement gland protein XAG-2 homolog antibodySecreted cement gland protein XAG2 homolog antibodyXAG 2 antibodyXAG2 antibody
see all
Anti-Anterior Gradient 2 antibody images
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Immunohistochemistry (Paraffin-embedded sections) - Anterior Gradient 2 antibody (ab43043)Immunohistochemistry of AGR2 in human tissues prepared from paraffin-embedded tissue blocks. Rabbit anti-AGR2 antibody ab43043 was used as primary antibody at a dilution range of 2 ug/ml to 4 ug/ml. -
Western blot - Anterior Gradient 2 antibody (ab43043)All lanes : Anti-Anterior Gradient 2 antibody (ab43043) at 2 µg/ml
Lane 1 : HeLa cell extract
Lane 2 : PC3 cell extract
Lysates/proteins at 20 µg per lane.
Predicted band size : 20 kDa -
Immunocytochemistry/ Immunofluorescence - Anterior Gradient 2 antibody (ab43043)This image is courtesy of an anonymous Abreviewab43043 staining cells from a human pancreatic cell line by ICC/IF. Cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 1% BSA for 30 minutes at 22°C. The primary antibody was diluted 1/350 and incubated with the sample for 1 hour at 22°C. An Alexa Fluor® 488 conjugated donkey anti-rabbit antibody, diluted 1/1000, was used as the secondary.
References for Anti-Anterior Gradient 2 antibody (ab43043)
This product has been referenced in:
- Ho ME et al. Prostate cancer cell phenotypes based on AGR2 and CD10 expression. Mod Pathol : (2013). Read more (PubMed: 23348903) »
- Dumartin L et al. AGR2 is a novel surface antigen that promotes the dissemination of pancreatic cancer cells through regulation of cathepsins B and D. Cancer Res 71:7091-102 (2011). WB ; Human . Read more (PubMed: 21948970) »
