Anti-SA1 antibody (ab4455)

Overview

• Product nameAnti-SA1 antibodySee all SA1 primary antibodies ...
• Description
  Goat polyclonal to SA1
• Tested applicationsWB, ICC, ICC/IF, IP more details
• Species reactivity
  Reacts with: Human, Xenopus laevis
  
• Immunogen

  Synthetic peptide (Human) - 36 amino acids which represent a portion of SA 1 encoded in part by exons 5 and 6.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.1% Sodium Azide
  Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Chromosome Structure
  • Chromatid Cohesion

  • Cell Biology
  • Cell Cycle
  • Cell Division
  • Chromatid Cohesion

Applications

Target

• FunctionComponent of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.
• Sequence similaritiesBelongs to the SCC3 family.
  Contains 1 SCD (stromalin conservative) domain.
• Post-translational
  modificationsPhosphorylated by PLK. The large dissociation of cohesin from chromosome arms during prophase is partly due to its phosphorylation.
• Cellular localizationNucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation.

Target information above from: UniProt accession Q8WVM7 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 10274 Human
  • Entrez Gene: 399163 Xenopus laevis
  • Omim: 604358 Human
  • SwissProt: Q8WVM7 Human
  • SwissProt: Q9DGN1 Xenopus laevis
  • Unigene: 412586 Human
  • Unigene: 3441 Xenopus laevis

• Alternative names
  Cohesin subunit SA 1 antibodyCohesin subunit SA-1 antibodyCohesin Subunit SA1 antibody

  condensin antibodyNuclear protein stromal antigen 1 antibodypds 5 antibodySA 1 antibodysa 2 antibodySCC 3 homolog 1 antibodySCC1 antibodyScc3 antibodySCC3 homolog 1 antibodySTAG 1 antibodystag1 antibodySTAG1_HUMAN antibodyStromal antigen 1 antibody

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Anti-SA1 antibody images

• 

  Western blot - SA1 antibody (ab4455)
  
  
  Predicted band size : 144 kDa
  
  
  Nuclear Extract from HeLa cells (~5 x 105 cells).
  
  ab4455 at 0.5 µg/ml.
  
  Detection: ECL with 15 second exposure.
• 

  Immunocytochemistry/ Immunofluorescence - SA1 antibody (ab4455)This image is courtesy of an Abreview submitted by Dr Kirk McManus
  Goat polyclonal to SA1 antibody (ab4455) at a 1/500 dilution detecting Stromal Antigen 1 in assynchronous HeLa cells. Cells were fixed with 4% paraformaldehyde and counterstained with DAPI. Secondary antibody:Donkey anti-goat IgG conjugated to Cy3. For further information please refer to abreview.

  See Abreview