Anti-MCM4 antibody (ab4459)

Overview

• Product nameAnti-MCM4 antibodySee all MCM4 primary antibodies ...
• Description
  Rabbit polyclonal to MCM4
• Tested applicationsICC/IF, WB more details
• Species reactivity
  Reacts with: Mouse, Human, Xenopus laevis
  Predicted to work with: Fruit fly (Drosophila melanogaster)
• Immunogen

  Synthetic peptide (Human) - 32 amino acids which represented a portion of human MCM 4 encoded within exon 14.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.1% Sodium Azide
  Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Synthesis
  • Other

Applications

Target

• FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
• Sequence similaritiesBelongs to the MCM family.
  Contains 1 MCM domain.
• Cellular localizationNucleus.

Target information above from: UniProt accession P33991 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 35679 Fruit fly (Drosophila melanogaster)
  • Entrez Gene: 4173 Human
  • Entrez Gene: 17217 Mouse
  • Omim: 602638 Human
  • SwissProt: Q26454 Fruit fly (Drosophila melanogaster)
  • SwissProt: P33991 Human
  • SwissProt: P49717 Mouse
  • Unigene: 21562 Fruit fly (Drosophila melanogaster)

  • Unigene: 460184 Human
  • Unigene: 1500 Mouse

  see all

• Alternative names
  19G antibodyAI325074 antibodyAU045576 antibody

  CDC 21 antibodyCDC 54 antibodyCDC21 antibodyCDC21 homolog antibodyCDC21, S. pombe, homolog of antibodyCDC54 antibodyCell division cycle 21, S. pombe, homolog of antibodyDNA replication licensing factor MCM 4 antibodyDNA replication licensing factor MCM4 antibodyhCdc 21 antibodyhCdc21 antibodyHomolog of S. pombe cell devision cycle 21 antibodyKIAA4003 antibodymcdc21 antibodyMCM 4 antibodyMCM 4 minichromosome maintenance deficient 4 antibodyMCM4 antibodyMCM4 minichromosome maintenance deficient 4 antibodyMCM4 minichromosome maintenance deficient 4, mitotin (S. cerevisiae) antibodyMCM4_HUMAN antibodyMcmd4 antibodyMGC33310 antibodyMinichromosome Maintenance 4 antibodyMinichromosome maintenance complex component 4 antibodyMinichromosome maintenance deficient (S. cerevisiae) 4 antibodyMinichromosome maintenance deficient 4 antibodyMinichromosome maintenance deficient 4 homolog (S. cerevisiae) antibodyMinichromosome maintenance, S. cerevisiae, homolog of, 4 antibodymKIAA4003 antibodyP1 CDC21 antibodyP1-CDC21 antibodyP1CDC21 antibody

  see all

Anti-MCM4 antibody images

• 

  Western blot - MCM4 antibody (ab4459)
  
  
  Predicted band size : 96.6 kDa
  
  
  HeLa cell RIPA extract incubated with 0.2 µg/ml of ab4459 for 1 hour.
• 

  Western blot - MCM4 antibody (ab4459)
  Anti-MCM4 antibody (ab4459) at 1/2000 dilution + HeLa nuclear cell lysate. at 20 µg
  
  Secondary
  Goat anti rabbit HRP. at 1/5000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 96.6 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence - MCM4 antibody (ab4459)
  ICC/IF image of ab4459 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4459, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.