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Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab4466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52.6 kDa).

Target

  • FunctionCatalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Directly acetylates and activates ATM. In case of HIV-1 infection, interaction with the viral Tat protein leads to KAT5 polyubiquitination and targets it to degradation.
  • Sequence similaritiesBelongs to the MYST (SAS/MOZ) family.
    Contains 1 C2HC-type zinc finger.
  • Post-translational
    modifications    Sumoylated by UBE2I at Lys-430 and Lys-451, leading to increase of its histone acetyltransferase activity in UV-induced DNA damage response, as well as its translocation to nuclear bodies.
    Phosphorylated on Ser-86 and Ser-90; enhanced during G2/M phase. Phosphorylated form has a higher activity.
    Ubiquitinated by MDM2, leading to its proteasome-dependent degradation.
  • Cellular localizationNucleus > nucleolus. Cytoplasm > perinuclear region. Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region and UV irradiation induces translocation into punctuate subnuclear structures named nuclear bodies.

    • Target information above from: UniProt accession Q92993 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        60 kDa Tat interactive protein antibody60 kDa Tat-interactive protein antibodycPLA(2) interacting protein antibody
        cPLA(2)-interacting protein antibodycPLA2 antibodycPLA2 interacting protein antibodyESA1 antibodyHistone acetyltransferase HTATIP antibodyHistone acetyltransferase KAT5 antibodyHIV 1 Tat interactive protein antibodyHIV 1 Tat interactive protein, 60kDa antibodyHIV-1 Tat interactive protein antibodyHTATIP antibodyHTATIP1 antibodyK(lysine) acetyltransferase 5 antibodyK-acetyltransferase 5 antibodyKAT5 antibodyKAT5_HUMAN antibodyLysine acetyltransferase 5 antibodyPLIP antibodyTat interacting protein, 60kDa antibodyTIP antibodyTip60 antibody
      see all

    Anti-KAT5 / Tip60 antibody images

    • Western blot - Anti-KAT5 / Tip60 antibody (ab4466)
      Western blot - Anti-KAT5 / Tip60 antibody (ab4466)


      Predicted band size : 52.6 kDa


      ab4466 works well on NuA4 purified fractions from yeast whole cell extracts. The NuA4 HAT complex was purified by tandem affinity (IgG and calmodulin) using a Epl1-TAP strain (another NuA4 subunit). 250ml yeast culture was used for the purification and 5ul of calmodulin elution (1/10 of the prep) was used in western with 1:1000 dilution of ab4466.

      Jacques Cote

    References for Anti-KAT5 / Tip60 antibody (ab4466)

    This product has been referenced in:
    • Ginsburg DS  et al. NuA4 lysine acetyltransferase Esa1 is targeted to coding regions and stimulates transcription elongation with Gcn5. Mol Cell Biol 29:6473-87 (2009). WB ; Saccharomyces cerevisiae . Read more (PubMed: 19822662) »
    • Decker PV  et al. Catalytic-Site Mutations in the MYST Family Histone Acetyltransferase Esa1. Genetics 178:1209-20 (2008). Read more (PubMed: 18245364) »

    See all 3 Publications for this product

    Publishing research using ab4466 ? Please let us know so that we can cite the reference in this datasheet

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    I never purchased this antibody so far, but since my group is working mainly on flotillins, I thought I would point you to the fact that there are no flotillins in S. cerevisiae. I am, however, interested in the other anti-flotillin Abs of Abcam and may c...

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    Thank you for your valuable comment. It is much appreciated.

    If you need any further assistance in the future, please do not hesitate to contact me.

    Inquiry: This product has been stated to work for Wb in s. cerevisiae... Unfortunately, flotillins DO NOT EXIST in this organism!!!! I think it might be helpfull for ignorant people to mention this fact as somebody might be mislead by the image shown.

    Thank you for your valuable comment.

    After discussing this enquiry with some colleagues, we regret to inform you that there was an error to set up the target of this antibody. We have amended the datasheet and will arrange a product recall to in...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"