Human CD14 ELISA Kit (ab45523)
Key features and details
- Sensitivity: 6 ng/ml
- Range: 6.89 ng/ml - 220 ng/ml
- Sample type: Cell culture supernatant, Cell Lysate, Milk, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CD14 ELISA Kit
See all CD14 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Sample A 8 210.5ng/ml 2.6 1.23% Sample B 8 23.4ng/ml 1.14 4.6% Inter-assay Sample n Mean SD CV% Sample A 8 210.5ng/ml 1.55 0.73% Sample B 8 24ng/ml 2 8.3% -
Sample type
Cell culture supernatant, Milk, Serum, Plasma, Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
< 6 ng/ml -
Range
6.89 ng/ml - 220 ng/ml -
Assay time
3h 45m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s CD14 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD14 in Human serum, plasma, buffered solutions or cell culture media.
A monoclonal antibody specific for CD14 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD14 concentrations and unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD14 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD14 present in the samples.
This kit will recognize both endogenous and recombinant Human CD14.
Get higher sensitivity in only 90 minutes with Human CD14 ELISA Kit (ab208983) from our SimpleStep ELISA® range.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 2 x 96 tests 1 x 96 tests 10X Standard Diluent Buffer Black 1 x 25ml 1 x 15ml 200X Wash Buffer White 2 x 10ml 1 x 10ml Biotinylated Antibody Diluent Red 1 x 13ml 1 x 7ml Biotinylated anti-CD14 2 x 0.3ml 1 x 0.3ml CD14 Microplate (12 x 8 well strips) 2 units 1 unit CD14 Standard (lyophilized) 4 vials 2 vials Chromogen TMB Substrate Solution 1 x 24ml 1 x 11ml HRP Diluent Red 1 x 23ml 1 x 12ml Stop Reagent Black 2 x 11ml 1 x 11ml Streptavidin-HRP 4 x 5ml 2 x 5ml -
Research areas
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Function
Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. -
Tissue specificity
Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages. -
Sequence similarities
Contains 11 LRR (leucine-rich) repeats. -
Post-translational
modificationsN- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. -
Cellular localization
Cell membrane. - Information by UniProt
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Alternative names
- CD 14
- CD_antigen=CD14
- CD14
see all -
Database links
- Entrez Gene: 929 Human
- Omim: 158120 Human
- SwissProt: P08571 Human
- Unigene: 163867 Human
Associated products
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SimpleStep ELISA kits
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab45523 has been referenced in 1 publication.
- Malherbe G et al. Circulating biomarkers of immune activation distinguish viral suppression from nonsuppression in HAART-treated patients with advanced HIV-1 subtype C infection. Mediators Inflamm 2014:198413 (2014). PubMed: 24808634