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IL6 High Sensitivity Human ELISA Kit (ab46044)

Abreviews (1)Q&A (7)Specific References (1)

Overview

  • Product nameIL6 High Sensitivity Human ELISA KitSee all IL6 kits ...
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.4%
    Inter-assay
    Sample n Mean SD CV%
    Sample 1 18 25pg/ml 3 12.2%
    Sample 2 18 12pg/ml 1 8.7%
    Sample 3 18 6pg/ml 1 13.7%
    Sample 4 18 41pg/ml 2 5.9%
    Sample 5 18 31pg/ml 1 4.9%
  • Tests
    2 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 0.8 pg/ml
  • Range
    1.56 pg/ml - 50 pg/ml
  • Recovery

    93 %

  • Assay time
    3h 45m
  • Species reactivity
    Reacts with: Human
  • Product overview

    The Human high sensitivity IL6 ELISA is to be used for the in-vitro quantitative determination of interleukin-6 (IL6) in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant human IL6. This kit has been configured for research use only and is not to be used in diagnostic procedures.

    Principal:

    The IL6 Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for IL6 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL6 concentrations, control specimens and unknowns are pipetted into these wells. During the first incubation, the IL6 antigen and a biotinylated monoclonal antibody specific for IL6 are simultaneously incubated. After washing, the enzyme ( streptavidin-peroxydase ) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL6 present in the samples.

     

    Recognizes both natural and recombinant human IL6

    Range: 1.56 pg/ml - 50 pg/ml

    Sensitivity <0.8 pg/ml

  • Notes

    Safety Notes:

    • For research use only.

    • The human blood components included in this kit have been tested and found non reactive for HBsAg and anti-HIV. Nevertheless, no known method can offer complete assurance that human blood derivatives will not transmit hepatitis, AIDS or other infections. Therefore, handling of reagents, serum or plasma specimens should be in accordance with local safety procedures , e.g. CDC/NIH Health manual : " Biosafety in Microbiological and Biomedical Laboratories" 1984.

    • Avoid any skin contact with H2SO4 and TMB. In case of contact, wash thoroughly with water.

    • Do not eat, drink, smoke or apply cosmetics where kit reagents are used.

    • Do not pipette by mouth.

    • The stop solution in this kit contains 9.8% sulphuric acid.

  • Tested applicationsELISA more details

Properties

  • FunctionCytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • Involvement in diseaseGenetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • Sequence similaritiesBelongs to the IL-6 superfamily.
  • Post-translational
    modifications    N- and O-glycosylated.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P05231 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        B cell differentiation factor B cell stimulatory factor 2B-cell stimulatory factor 2
        BSF 2BSF-2BSF2CDFCTL differentiation factorCytotoxic T cell differentiation factor Hepatocyte stimulating factHGFHPGFHSF Hybridoma growth factorHybridoma plasmacytoma growth factor IFN-beta-2IFNB2 IL 6IL-6IL6IL6 protein IL6_HUMANInterferon beta 2Interferon beta-2Interleukin 6Interleukin 6 (interferon beta 2) Interleukin BSF 2Interleukin-6Interleukin6
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab46044 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      IL6 High Sensitivity Human ELISA Kit images

      • ELISA - High Sensitivity Human IL6 ELISA Kit (ab46044)
        ELISA - High Sensitivity Human IL6 ELISA Kit (ab46044)
        Typical IL6 standard curve ranging from 1.56 to 50 pg/mL.

      References for IL6 High Sensitivity Human ELISA Kit (ab46044)

      This product has been referenced in:
      • Kolkhof P  et al. Cardiac glycosides potently inhibit C-reactive protein synthesis in human hepatocytes. Biochem Biophys Res Commun 394:233-9 (2010). Read more (PubMed: 20206126) »

      See 1 Publication for this product

      Publishing research using ab46044 ? Please let us know so that we can cite the reference in this datasheet

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      ELISA abreview for IL6 High Sensitivity Human ELISA Kit

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      Abreviews
      Application ELISA
      Sample Human Cell (Lung)
      Specification Lung
      Amount of Sample growth media µg
      Type of Kit Used ELISA
      Positive control Supplied with kit
      Did you use the Abcam Kit protocol? Yes
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      Submitted Feb 11 2009

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"