IL6 High Sensitivity Human ELISA Kit (ab46044)
- Product nameIL6 High Sensitivity Human ELISA KitSee all IL6 kits ...
Intra-assay Sample n Mean SD CV% Overall 4.4% Inter-assay Sample n Mean SD CV% Sample 1 18 25pg/ml 3 12.2% Sample 2 18 12pg/ml 1 8.7% Sample 3 18 6pg/ml 1 13.7% Sample 4 18 41pg/ml 2 5.9% Sample 5 18 31pg/ml 1 4.9%
- Tests2 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 0.8 pg/ml
- Range1.56 pg/ml - 50 pg/ml
- Assay time3h 45m
- Species reactivityReacts with: Human
- Product overview
The Human high sensitivity IL6 ELISA is to be used for the in-vitro quantitative determination of interleukin-6 (IL6) in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant human IL6. This kit has been configured for research use only and is not to be used in diagnostic procedures.
The IL6 Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for IL6 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL6 concentrations, control specimens and unknowns are pipetted into these wells. During the first incubation, the IL6 antigen and a biotinylated monoclonal antibody specific for IL6 are simultaneously incubated. After washing, the enzyme ( streptavidin-peroxydase ) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL6 present in the samples.
Recognizes both natural and recombinant human IL6
Range: 1.56 pg/ml - 50 pg/ml
Sensitivity <0.8 pg/ml
- For research use only.
- The human blood components included in this kit have been tested and found non reactive for HBsAg and anti-HIV. Nevertheless, no known method can offer complete assurance that human blood derivatives will not transmit hepatitis, AIDS or other infections. Therefore, handling of reagents, serum or plasma specimens should be in accordance with local safety procedures , e.g. CDC/NIH Health manual : " Biosafety in Microbiological and Biomedical Laboratories" 1984.
- Avoid any skin contact with H2SO4 and TMB. In case of contact, wash thoroughly with water.
- Do not eat, drink, smoke or apply cosmetics where kit reagents are used.
- Do not pipette by mouth.
- The stop solution in this kit contains 9.8% sulphuric acid.
- Tested applicationsELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 25ml 200X Washing Buffer White 2 x 10ml Biotinylated Antibody Diluent Red 1 x 13ml Biotinylated anti-IL-6 Red 2 x 0.4ml Chromogen TMB 1 x 24ml HRP Diluent Red 1 x 23ml IL-6 Control Silver 4 vials IL-6 Microplate 8 units IL-6 Standard Yellow 4 vials Standard Diluent (human serum) 2 vials Stop Reagent Black 2 x 11ml Streptavidin-HRP 4 x 5µl
- Research Areas
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
modificationsN- and O-glycosylated.
- B cell differentiation factor B cell stimulatory factor 2B-cell stimulatory factor 2
- BSF 2BSF-2BSF2CDFCTL differentiation factorCytotoxic T cell differentiation factor Hepatocyte stimulating factHGFHPGFHSF Hybridoma growth factorHybridoma plasmacytoma growth factor IFN-beta-2IFNB2 IL 6IL-6IL6IL6 protein IL6_HUMANInterferon beta 2Interferon beta-2Interleukin 6Interleukin 6 (interferon beta 2) Interleukin BSF 2Interleukin-6Interleukin6
Our Abpromise guarantee covers the use of ab46044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IL6 High Sensitivity Human ELISA Kit images
Typical IL6 standard curve ranging from 1.56 to 50 pg/mL.
References for IL6 High Sensitivity Human ELISA Kit (ab46044)
This product has been referenced in:
- Kolkhof P et al. Cardiac glycosides potently inhibit C-reactive protein synthesis in human hepatocytes. Biochem Biophys Res Commun 394:233-9 (2010). Read more (PubMed: 20206126) »