IL1 alpha Human ELISA Kit (ab46051)

Overview

• Product nameIL1 alpha Human ELISA KitSee all IL1 alpha kits ...
• Detection methodColorimetric
• Precision

  Intra-assay

  Sample	n	Mean	SD	CV%
  A	22	459.6pg/ml	13	2.8%
  B	21	72.6pg/ml	3.6	4.3%

  Inter-assay

  Sample	n	Mean	SD	CV%
  A	8	259.9pg/ml	15	5.8%
  B	8	58.6pg/ml	4.3	7.3%

• Tests
  2 x 96 well plate
• Sample type
  Cell culture supernatant, Serum, Plasma
• Assay typeSandwich
• Sensitivity
  < 10 pg/ml
• Range
  31.2 pg/ml - 1000 pg/ml
• Recovery

  95.3 %
  

  Sample specific recovery

  Sample type	Average %	Range
  Serum	95.3	31.25pg/ml - 1000pg/ml

• Assay time
  3h 45m
• Assay durationMultiple steps standard assay
• Species reactivity
  Reacts with: Human
  
• Product overview

  The Human IL1 alpha ELISA is to be used for the in-vitro quantitative determination of interleukin-1 alpha (IL1 alpha) in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant human IL1 alpha. This kit has been configured for research use only and is not to be used in diagnostic procedures.

  Principal:

  The IL1 alpha Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for IL1 alpha has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL1 alpha concentrations and unknowns are pipetted into these wells. During the first incubation, the IL1 alpha antigen is added to wells. After washing, a biotinylated monoclonal antibody specific for IL1 alpha is incubated. Then the enzyme ( streptavidin-peroxydase ) is added. After incubation and washing to remove all unbound enzyme, a substrate solution which acts on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL1 alpha present in the samples.

   

  Recognizes both natural and recombinant human IL1 alpha
  
  Range: 31.2 pg/ml - 1000 pg/ml
  
  Sensitivity <10 pg/ml

• Notes

  Safety Notes:

  • For research use only.
  • The human blood components included in this kit have been tested and found non reactive for HBsAg and anti-HIV. Nevertheless, no known method can offer complete assurance that human blood derivatives will not transmit hepatitis, AIDS or other infections. Therefore, handling of reagents, serum or plasma specimens should be in accordance with local safety procedures , e.g. CDC/NIH Health manual : " Biosafety in Microbiological and Biomedical Laboratories" 1984.
  • Avoid any skin contact with H₂SO₄ and TMB. In case of contact, wash thoroughly with water.
  • Do not eat, drink, smoke or apply cosmetics where kit reagents are used.
  • Do not pipette by mouth.
  • The stop solution in this kit contains 9.8% sulphuric acid.

• Tested applicationsELISA more details
• PlatformMicroplate

Properties

• Storage instructionsStore at +4°C. Please refer to protocols.

• Components	Identifier	2 x 96 tests
  10X Standard Diluent Buffer	Black	1 x 25ml
  200X Washing Buffer	White	2 x 10ml
  Biotinylated Antibody Diluent	Red	1 x 13ml
  Biotinylated anti-IL1α	Red	2 x 0.4ml
  Chromogen TMB		1 x 24ml
  HRP Diluent	Red	1 x 23ml
  IL1α Control	Silver	4 vials
  IL1α precoated microtiter plates		8 units
  IL1α Standard	Yellow	4 vials
  Stop Reagent	Black	2 x 11ml
  Streptavidin-HRP		4 x 5µl

• Research Areas
  • Cancer
  • Tumor immunology
  • Cytokines
  • Interleukins

  • Kits/ Lysates/ Other
  • Kits
  • ELISA Kits
  • ELISA Kits
  • Cytokines and cytokine receptors ELISA kits

  • Immunology
  • Innate Immunity
  • Cytokines
  • Interleukins

Applications

IL1 alpha Human ELISA Kit images

• 

  ELISA - Human IL1 alpha ELISA Kit (ab46051)
  Typical IL1 alpha standard curve ranging from 1000 to 31.25 pg/mL.

Protocols

• Human IL1 alpha ELISA Kit