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Overview

  • Product nameGM-CSF Murine ELISA KitSee all GM-CSF kits ...
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    A 8 279.2pg/ml 14.7 5.2%
    B 8 62.5pg/ml 2.52 4%
    Inter-assay
    Sample n Mean SD CV%
    A 7 271.9pg/ml 10.8 3.9%
    B 7 65.4pg/ml 4.3 6.5%
  • Tests
    2 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum
  • Assay typeSandwich
  • Sensitivity
    < 9 pg/ml
  • Range
    15.6 pg/ml - 500 pg/ml
  • Assay time
    3h 45m
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    The Murine GM-CSF ELISA is to be used for the in-vitro quantitative determination of murine GM-CSF (mGM-CSF) in murine serum, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant mGM-CSF.

    Principal:

    The mGM-CSF Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for mGM-CSF has been coated onto the wells of the microtiter strips provided. The antigen and a biotinylated monoclonal antibody specific for mGM-CSF are simultaneously incubated. Revelation step includes Streptavidin-Horse Radish peroxidase and TMB as chromogen.

     

    Recognizes both natural and recombinant Murine GM-CSF

    Range: 15.6 pg/ml - 500 pg/ml

    Sensitivity < 9 pg/ml

  • Notes

    Safety Notes:

    • For research use only.

    • The human blood components included in this kit have been tested and found non reactive for HBsAg and anti-HIV. Nevertheless, no known method can offer complete assurance that human blood derivatives will not transmit hepatitis, AIDS or other infections. Therefore, handling of reagents, serum or plasma specimens should be in accordance with local safety procedures , e.g. CDC/NIH Health manual : " Biosafety in Microbiological and Biomedical Laboratories" 1984.

    • Avoid any skin contact with H2SO4 and TMB. In case of contact, wash thoroughly with water.

    • Do not eat, drink, smoke or apply cosmetics where kit reagents are used.

    • Do not pipette by mouth.

    • The stop solution in this kit contains 9.8% sulphuric acid.

  • Tested applicationsELISA more details

Properties

  • RelevanceGM-CSF is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of GM-CSF is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.
  • Cellular localizationSecreted
    • Alternative names
        Colony stimulating factor Colony stimulating factor 2 (granulocyte macrophage) CSF
        CSF 2CSF2GMCSF Granulocyte macrophage colony stimulating factorMGC131935MGC138897Molgramostin Sargramostim
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab46119 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      GM-CSF Murine ELISA Kit images

      • ELISA - Murine GM-CSF ELISA Kit (ab46119)
        ELISA - Murine GM-CSF ELISA Kit (ab46119)
        Typical mGM-CSF standard curve ranging from 15.6 to 500 pg/mL.

      References for GM-CSF Murine ELISA Kit (ab46119)

      ab46119 has not yet been referenced specifically in any publications.

      Publishing research using ab46119 ? Please let us know so that we can cite the reference in this datasheet

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"