Anti-c Abl antibody (ab47410)

Overview

• Product nameAnti-c Abl antibodySee all c Abl primary antibodies ...
• Description
  Rabbit polyclonal to c Abl
• SpecificityThis antibody detects endogenous levels of total c-Abl protein.
• Tested applicationsSandwich ELISA, IHC-P, ELISA more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  The antiserum was produced against synthesized non-phosphopeptide derived from human c-Abl around the phosphorylation site of tyrosine 412 (D-T-Y^P-T-A).

• Positive controlHuman breast carcinoma tissue

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesThis antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Oncoproteins/suppressors
  • Oncoproteins
  • Signal transducers

  • Signal Transduction
  • Protein Phosphorylation
  • Tyrosine Kinases
  • Other

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Cancer susceptibility
  • Proto-oncogenes

Applications

Target

• FunctionProtein kinase that regulates key processes linked to cell growth and survival. Regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. Localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks.
• Tissue specificityWidely expressed.
• Involvement in diseaseNote=A chromosomal aberration involving ABL1 is a cause of chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with BCR. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).
• Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily.
  Contains 1 protein kinase domain.
  Contains 1 SH2 domain.
  Contains 1 SH3 domain.
• Post-translational
  modificationsPhosphorylated by PRKDC (By similarity). DNA damage-induced activation of c-Abl requires the function of ATM and Ser-446 phosphorylation (By similarity). Phosphorylation on Thr-735 is required for binding 14-3-3 proteins for cytoplasmic translocation.
  Isoform IB is myristoylated on Gly-2.
• Cellular localizationCytoplasm > cytoskeleton. Nucleus. Sequestered into the cytoplasm through interaction with 14-3-3 proteins and Nucleus membrane. The myristoylated c-ABL protein is reported to be nuclear.

Target information above from: UniProt accession P00519 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 25 Human
  • Entrez Gene: 11350 Mouse
  • Omim: 189980 Human
  • SwissProt: P00519 Human
  • SwissProt: P00520 Mouse
  • Unigene: 431048 Human
  • Unigene: 1318 Mouse
  • Unigene: 474779 Mouse

• Alternative names
  Abelson Murine Leukemia Viral Oncogene Homolog 1 antibodyAbelson murine leukemia viral v abl oncogene homolog 1 antibodyAbelson tyrosine protein kinase 1 antibody

  Abl 1 antibodyABL antibodyAbl protein antibodyAbl1 antibodyABL1_HUMAN antibodybcr/abl antibodyBcr/c abl oncogene protein antibodyc abl oncogene 1 non receptor tyrosine kinase antibodyc abl oncogene 1 receptor tyrosine kinase antibodyJTK 7 antibodyJTK7 antibodyp150 antibodyProto oncogene tyrosine protein kinase ABL1 antibodyProto-oncogene c-Abl antibodyTransformation gene oncogene ABL antibodyTyrosine-protein kinase ABL1 antibodyv abl Abelson murine leukemia viral oncogene homolog 1 antibodyv abl antibody

  see all

Anti-c Abl antibody images

• 

  Immunohistochemistry (Paraffin-embedded sections) - c Abl antibody (ab47410)
  Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue in the presence (right) and absence (left) of blocking peptide, using ab47410 at 1/50 dilution.