Anti-CDK1 (phospho Y15) antibody (ab47594)
Overview
- Product nameAnti-CDK1 (phospho Y15) antibodySee all CDK1 primary antibodies ...
- DescriptionRabbit polyclonal to CDK1 (phospho Y15)
- SpecificityThis antibody is specific for Cdc2.
- Tested applicationsWB, ELISA more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
A synthesized phosphopeptide derived from human Cdc2 around the phosphorylation site of tyrosine 15 (G-T-YP-G-V)
- Positive controlHepG2 cell extract.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab47594 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa). |
| ELISA | ELISA: 1/10000. |
Target
- FunctionPlays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
- Tissue specificityIsoform 2 is found in breast cancer tissues.
- Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. - Cellular localizationNucleus.
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Database links
- Entrez Gene: 983 Human
- Entrez Gene: 983 Human
- Entrez Gene: 983 Human
- Entrez Gene: 12534 Mouse
- Entrez Gene: 54237 Rat
- Omim: 116940 Human
- SwissProt: P06493 Human
- SwissProt: P06493 Human
- SwissProt: P06493 Human
- SwissProt: P11440 Mouse
- SwissProt: P39951 Rat
- Unigene: 334562 Human
- Unigene: 281367 Mouse
- Unigene: 6934 Rat
see all
Target information above from: UniProt accession
P06493
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Cdc 2 antibodyCdc2 antibodyCDC28A antibody
- CDK 1 antibodyCDK1 antibodyCDK1_HUMAN antibodyCDKN1 antibodyCELL CYCLE CONTROLLER CDC2 antibodyCell division control protein 2 antibodyCell division control protein 2 homolog antibodyCell division cycle 2 G1 to S and G2 to M antibodyCell division protein kinase 1 antibodyCell Divsion Cycle 2 Protein antibodyCyclin Dependent Kinase 1 antibodyCyclin-dependent kinase 1 antibodyDKFZp686L20222 antibodyMGC111195 antibodyp34 Cdk1 antibodyp34 Protein Kinase antibodyP34CDC2 antibody
see all
Anti-CDK1 (phospho Y15) antibody images
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Western blot - Cdc2 (phospho Y15) antibody (ab47594)Anti-CDK1 (phospho Y15) antibody (ab47594) at 1/500 dilution + HepG2 cell extract
Predicted band size : 34 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
References for Anti-CDK1 (phospho Y15) antibody (ab47594)
This product has been referenced in:
- Caretti V et al. WEE1 kinase inhibition enhances the radiation response of diffuse intrinsic pontine gliomas. Mol Cancer Ther 12:141-50 (2013). WB ; Mouse . Read more (PubMed: 23270927) »
- Szilagyi Z et al. Cyclin-dependent kinase 8 regulates mitotic commitment in fission yeast. Mol Cell Biol 32:2099-109 (2012). WB ; Schizosaccharomyces pombe . Read more (PubMed: 22451489) »
