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IL10 Human Elipair Kit (Reagents for 10 x 96 Well Plates) (ab48486)

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Overview

  • Product nameIL10 Human Elipair Kit (Reagents for 10 x 96 Well Plates)See all IL10 kits ...
  • Tests
    10 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeELIPAIR
  • Sensitivity
    < 5 pg/ml
  • Range
    400 pg/ml - 12.5 pg/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    The IL10 Eli-pair includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

    This Eli-pair set is intended to measure human IL10 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

     

    Sensitivity: < 5 pg / ml

  • Tested applicationsELISA more details
  • PlatformReagents

Properties

  • FunctionInhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • Tissue specificityProduced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
  • Sequence similaritiesBelongs to the IL-10 family.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P22301 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        CSIFCytokine synthesis inhibitory factorIL-10
        IL10IL10_HUMANInterleukin 10Interleukin-10TGIF
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab48486 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA
    • Application notesELISA: Use at an assay dependent dilution.


      Not yet tested in other applications.
      Optimal dilutions/concentrations should be determined by the end user.

      • IL10 Human Elipair Kit (Reagents for 10 x 96 Well Plates) images

      • Eli-pair - IL10 Human Elipair Kit (Reagents for 10 x 96 Well Plates) (ab48486)
        Eli-pair - IL10 Human Elipair Kit (Reagents for 10 x 96 Well Plates) (ab48486)
        IL-10 Standard curve ranging from 12.5 to 400 pg/mL

      References for IL10 Human Elipair Kit (Reagents for 10 x 96 Well Plates) (ab48486)

      ab48486 has not yet been referenced specifically in any publications.

      Publishing research using ab48486 ? Please let us know so that we can cite the reference in this datasheet

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"