Overview
Product nameHIV1 Nef protein (Tagged)See all HIV1 Nef proteins and peptides ...
Protein descriptionRecombinant fusion fragment;HIV1 nef (amino acids 3 to 190 M.Wt ~ 20kDa) fused to ß-galactosidase (114 kDa) at the N-terminus.
Expression hostE. coli
Properties
Purity> 95
% by SDS-PAGE
Biological activityReacts strongly with human HIV positive serum.
FormLiquid
Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: None
Constituents: 0.014M Beta mercaptoethanol, 0.01M Sodium carbonate, 0.01M EDTA
Concentration information loading...
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab49060
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|
Application
|
Notes |
| ELISA |
ELISA: Use at an assay dependent dilution. |
| WB |
WB: Use at an assay dependent dilution. |
Protein info
-
Alternative names
C terminal core proteinF proteinNef
see all
RelevanceNef is a early protein that appears to play a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. Nef enhances virus infectivity and pathogenicity. It down modulates surface MHC I molecules and internalized molecules are sequested to the trans-Golgi network. The number of cell surface CD4 antigen are decreased by interacting with the Src family kinase LCK thereby inducing LCK CD4 dissociation and by increasing clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation.
References for HIV1 Nef protein (Tagged) (ab49060)
ab49060
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
