Overview
- Product nameAnti-Neomycin Phosphotransferase 2 antibodySee all Neomycin Phosphotransferase 2 primary antibodies ...
- DescriptionRabbit polyclonal to Neomycin Phosphotransferase 2
- Specificityab49975 recognizes bacterial Neomycin Phosphotransferase 2 expressed in transgenic tobacco plants.
- Tested applicationsWB more details
- Species reactivityReacts with: Escherichia coli
- Immunogen
Synthetic peptide: AIPVSECSVQQWTTHAGL conjugated to KLH, corresponding to internal sequence amino acids 133-150 of Escherichia coli Neomycin Phosphotransferase 2
- Positive controlCytosolic fraction from the leaves of transgenic Nicotiana tabacum expressing E. coli Neomycin Phosphotransferase 2.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab49975 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 29 kDa. |
Target
- RelevanceNeomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin®, G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids.
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Database links
- SwissProt: P14509 Escherichia coli
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Alternative names
- Aminoglycoside 3' phosphotransferase antibodyAPH(3') II antibodyAPH(3')II antibody
- aphA antibodyEC 2.7.1.95 antibodyKan antibodyKanamycin kinase type II antibodyKanamycin kinase, type VIII antibodykanamycin resistance gene antibodyNeo antibodyNeomycin kanamycin phosphotransferase type II antibodyNeomycin kanamycin phosphotransferase type VIII antibodyNeomycin phosphotransferase II antibodyNeomycine phosphotransferase antibodyNPT II antibodyNPTII antibody
see all
Anti-Neomycin Phosphotransferase 2 antibody images
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Western blot - Neomycin Phosphotransferase 2 antibody (ab49975)Anti-Neomycin Phosphotransferase 2 antibody (ab49975) at 1/500 dilution + Whole cell lysate prepared from SW48 cancer cell line at 20 µg
Secondary
Goat anti-rabbit conjugated to HRP at 1/2000 dilution
developed using the ECL technique
Predicted band size : 29 kDa
Observed band size : 29 kDa
Exposure time : 5 minutesImage courtesy of an anonymous Abreview.
References for Anti-Neomycin Phosphotransferase 2 antibody (ab49975)
ab49975 has not yet been referenced specifically in any publications.
