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GDF8 / Myostatin protein (ab50097)

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Overview

  • Product nameGDF8 / Myostatin proteinSee all GDF8 / Myostatin proteins and peptides ...
  • Protein descriptionRecombinant Human Myostatin; a 25.0 kDa protein consisting of two identical 109 amino acid polypeptides linked by a single disulfide bond.
  • Expression hostE. coli

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Purification notesGreater than 98% by SDS-PAGE and HPLC analyses.
  • FormLyophilised:Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • Concentration information loading...
  • Sequence notesDFGLDCDEHS TESRCCRYPL TVDFEAFGWD WIIAPKRYKA NYCSGECEFV FLQKYPHTHL VHQANPRGSA GPCCTPTKMS PINMLYFNGK EQIIYGKIPA MVVDRCGCS
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab50097 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Inhibition Assay Inhib: Use at an assay dependent dilution.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
    Functional Studies FuncS: Use at an assay dependent dilution. The inhibition of the proliferation of MPC-11 cells. The expected ED50 for this effect is 17.0-25.0 ng/ml.
  • Application notesThis peptide can be used with studies using ab98337.

    • Protein info

    • Alternative names
        GDF 8GDF-8GDF8_HUMAN
        Growth differentiation factor 8Growth/Differentiation Factor 8MSTNmyostatinOTTHUMP00000163498
      see all
  • FunctionActs specifically as a negative regulator of skeletal muscle growth.
  • Sequence similaritiesBelongs to the TGF-beta family.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession O14793 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for GDF8 / Myostatin protein (ab50097)

    ab50097 has not yet been referenced specifically in any publications.

    Publishing research using ab50097 ? Please let us know so that we can cite the reference in this datasheet

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    Submit a Question for ab50097

    Displaying 1 - 2 of 2 results for Q&A

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    Hi,



    I would like to purchase myostatin for a functional study in cells. I saw you have GDF8 / Myostatin protein (Active) ab73251 and http://www.abcam.com/gdf8-myostatin-protein-ab50097.html. What is the difference between these two prod...

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    Thank you for contacting us.

    These two proteins are the same, except for the reconstitution instructions (water versus 20 mM Hcl). Both should work equally well in inhiibiting MPC-11 cell growth.

    I hope this information is helpful to you...

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    Please provide a protocol for the inhibition assay this antibody has been tested in.

    Thank you for your recent telephone enquiry. I am pleased to confirm I have obtained the original testing procedure for inhibition assays using ab50097 GDF8 / Myostatin protein. Cytotoxicity Assay for h-Myostatin using MPC-11 cells Procedure: ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"