Overview
Product nameFGF6 protein
Protein descriptionRecombinant fragment of Human FGF6, a 18.7 kDa protein consisting of 168 amino acid residues
Expression hostE. coli
Properties
Purity> 95
% by SDS-PAGE
Purification notesGreater than 95% by SDS-PAGE.
FormLyophilised:Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use. Repeated freeze thaw cycles will result in some loss of activity.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: None
Constituents: 50mM Sodium chloride, 10mM Sodium phosphate, pH 7.2
Endotoxin level is less than 0.1 ng per µg (1EU/µg).
Concentration information loading...
Sequence notesGTRANNTLL DSRGWGTLLS RSRAGLAGEI AGVNWESGYL VGIKRQRRLY CNVGIGFHLQ VLPDGRISGT HEENPYSLLE ISTVERGVVS LFGVRSALFV AMNSKGRLYA TPSFQEECKF RETLLPNNYN AYESDLYQGT YIALSKYGRV KRGSKVSPIM TVTHFLPRI
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab50130
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|
Application
|
Notes |
| SDS-PAGE |
|
| Functional Studies |
|
Application notesFuncS: Use at an assay dependent dilution.
Biological Activity: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors is < 0.5 ng/ml, corresponding to a specific activity of > 2 x 106 units/mg.
SDS-PAGE: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
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Alternative names
FGF 6Fibroblast growth factor 6Fibroblast growth factor 6 precursor
see all
RelevanceFGF6 is a secreted heparin binding growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF6 is expressed in leukemia cell lines with platelet megakaryocytic differentiation potential. It signals through FGFR 1c, 2c, and 4.
The FGF6 gene displays oncogenic transforming activity when transfected into mammalian cells. The mouse homolog of this gene exhibits a restricted expression profile predominantly in the myogenic lineage, which suggested a role in muscle regeneration or differentiation.
Cellular localizationSecreted
References for FGF6 protein (ab50130)
ab50130
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
