Anti-Twist antibody (ab50581)
- Product nameAnti-Twist antibodySee all Twist primary antibodies ...
- DescriptionRabbit polyclonal to Twist
- Tested applicationsEMSA, IHC-P, WB, ICC/IF more details
- Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Chicken, Chimpanzee
Synthetic peptide: PADDSLSNSEEEPDRQ conjugated to KLH by a C-terminal Cysteine residue linker, corresponding to amino acids 12-27 of Human Twist
- Positive controlOsteosarcoma MG-63 cells. Human melanoma Mel15 cells. 293T cells expressing recombinant human Twist1.
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
- Concentration information loading...
- PurityImmunogen affinity purified
- Clonality Polyclonal
Our Abpromise guarantee covers the use of ab50581 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|EMSA||EMSA: Use at an assay dependent concentration. PubMed: 21931630|
|WB||WB: Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 21 kDa).|
|ICC/IF||ICC/IF: Use a concentration of 1 - 2 µg/ml.|
- FunctionActs as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation.
- Tissue specificitySubset of mesodermal cells.
- Involvement in diseaseDefects in TWIST1 are a cause of Saethre-Chotzen syndrome (SCS) [MIM:101400]; also known as acrocephalosyndactyly type 3 (ACS3). SCS is a craniosynostosis syndrome characterized by coronal synostosis, brachycephaly, low frontal hairline, facial asymmetry, hypertelorism, broad halluces, and clinodactyly.
Defects in TWIST1 are the cause of Robinow-Sorauf syndrome (RSS) [MIM:180750]; also known as craniosynostosis-bifid hallux syndrome. RSS is an autosomal dominant defect characterized by minor skull and limb anomalies which is very similar to Saethre-Chotzen syndrome.
Defects in TWIST1 are the cause of craniosynostosis type 1 (CRS1) [MIM:123100]. Craniosynostosis consists of premature fusion of one or more cranial sutures, resulting in an abnormal head shape.
- Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
- Cellular localizationNucleus.
- ACS3 antibodyB-HLH DNA binding protein antibodybHLHa38 antibody
- BPES2 antibodyBPES3 antibodyClass A basic helix-loop-helix protein 38 antibodyCRS1 antibodyH-twist antibodyOTTHUMP00000116043 antibodySCS antibodyTWIST antibodyTwist homolog 1 (Drosophila) antibodyTwist homolog 1 antibodyTWIST homolog of drosophila antibodyTwist related protein 1 antibodyTwist-related protein 1 antibodyTwist1 antibodyTWST1_HUMAN antibody
Anti-Twist antibody images
Human melanoma Mel15 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.1% Triton X-100. Fixed cells were stained with 2 µg/ml ab50581. The antibody was developed with Goat Anti-Rabbit IgG, Cy3 conjugate.
ab50581 staining Twist in murine brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using citric acid buffer pH 6.1. Samples were then blocked with 0.5% BSA for 30 minutes at room temperature followed by incubation with the primary antibody at a 1/500 dilution for 2 hours. A biotin-conjugated secondary antibody was used at a 1/100 dilution.
ab50581 staining Twist in human glioblastoma cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton X-100 and then blocked using 0.5% BSA for 20 minutes. Samples were then incubated with primary antibody at 1/50 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Cy3® used at a 1/400 dilution.
References for Anti-Twist antibody (ab50581)
This product has been referenced in:
- Chen Z et al. The Iron Chelators Dp44mT and DFO Inhibit TGF-ß-Induced Epithelial-Mesenchymal Transition via Up-Regulation of N-myc Downstream Regulated Gene 1 (NDRG1). J Biol Chem : (2012). WB . Read more (PubMed: 22453918) »
- Dave N et al. Functional cooperation between Snail1 and twist in the regulation of ZEB1 expression during epithelial to mesenchymal transition. J Biol Chem 286:12024-32 (2011). WB ; Mouse . Read more (PubMed: 21317430) »