Anti-14-3-3 zeta antibody (ab51129)

Overview

• Product nameAnti-14-3-3 zeta antibodySee all 14-3-3 zeta primary antibodies ...
• Description
  Rabbit polyclonal to 14-3-3 zeta
• Specificityab51129 detects endogenous levels of total 14-3-3 zeta protein.
• Tested applicationsELISA, WB, ICC, ICC/IF, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthesized non-phosphopeptide derived from human 14-3-3 zeta around the phosphorylation site of serine 58 (R-S-S^P-W-R).

• Positive controlHuman breast carcinoma tissue or extracts from 293 cells treated with Forskolin.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Adapters
  • Cytoplasmic

  • Tags & Cell Markers
  • Cell Type Markers
  • Tumor Associated

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Alzheimer's disease
  • Other

  • Cell Biology
  • Cell Cycle
  • Cell Division
  • Other cell division antibodies

  • Cell Biology
  • Apoptosis
  • Intracellular
  • Associated Proteins

Applications

Target

• FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
• Sequence similaritiesBelongs to the 14-3-3 family.
• Post-translational
  modificationsThe delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1.
• Cellular localizationCytoplasm. Melanosome. Located to stage I to stage IV melanosomes.

Target information above from: UniProt accession P63104 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 7534 Human
  • Entrez Gene: 22631 Mouse
  • Entrez Gene: 25578 Rat
  • Omim: 601288 Human
  • SwissProt: P63104 Human
  • SwissProt: P63101 Mouse
  • SwissProt: P63102 Rat
  • Unigene: 492407 Human

  • Unigene: 3360 Mouse
  • Unigene: 465895 Mouse
  • Unigene: 1292 Rat

  see all

• Alternative names
  14 3 3 delta antibody14 3 3 protein delta antibody14 3 3 protein zeta/delta antibody

  14 3 3 protein/cytosolic phospholipase A2 antibody14 3 3 zeta antibody14-3-3 protein zeta/delta antibody1433Z_HUMAN antibodyKCIP 1 antibodyKCIP-1 antibodyKCIP1 antibodyMGC111427 antibodyMGC126532 antibodyMGC13815 antibodyMGC138156 antibodyPhospholipase A2 antibodyProtein kinase C inhibitor protein 1 antibodyTyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein zeta polypeptide antibodyTyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, delta polypeptide antibodyTyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta polypeptide antibodyTyrosine 3/tryptophan 5 monooxygenase activation protein, zeta polypeptide antibodyYWHAD antibodyYWHAZ antibody

  see all

Anti-14-3-3 zeta antibody images

• 

  Immunohistochemistry (Paraffin-embedded sections) - 14-3-3 antibody (ab51129)
  This image shows paraffin-embedded human breast carcinoma tissue stained with ab51129 at a dilution of 1/100. Right hand image: tissue treated with immunising peptide; left hand image: untreated tissue
• 

  Western blot - 14-3-3 zeta antibody (ab51129)
  All lanes : Anti-14-3-3 zeta antibody (ab51129)
  
  Lane 1 : Extracts from 293 cells treated with Forskolin (40nM, 30min) with no peptide
  Lane 2 : Extracts from 293 cells treated with Forskolin (40nM, 30min) with immunising peptide
  
  
  Predicted band size : 28 kDa
  Observed band size : 28 kDa
  
• 

  Western blot
  All lanes : Anti-14-3-3 zeta antibody (ab51129) at 1/500 dilution
  
  Lane 1 : 14-3-3 zeta protein (Human) (ab85268) at 0.1 µg
  Lane 2 : 14-3-3 zeta protein (Human) (ab85268) at 0.01 µg
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Exposure time : 4 minutes
• 

  Immunocytochemistry/ Immunofluorescence - 14-3-3 zeta antibody (ab51129)
  ICC/IF image of ab51129 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51129, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.