Anti-MST3 antibody [EP1468Y] (ab51137)

Overview

• Product nameAnti-MST3 antibody [EP1468Y]See all MST3 primary antibodies ...
• Description
  Rabbit monoclonal [EP1468Y] to MST3
• Tested applicationsFlow Cyt, IP, WB, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide corresponding to residues on the C-terminus of human MST3.

• Positive controlHeLa cells, human brain tissue.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• Concentration information loading...
• Clonality Monoclonal
• Clone numberEP1468Y
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • Other Kinases

  • Neuroscience
  • Neurotransmission
  • Intracellular Signaling
  • Kinases

Applications

Target

• FunctionSerine/threonine-protein kinase that acts on both serine and threonine residues and promotes apoptosis in response to stress stimuli and caspase activation. Mediates oxidative-stress-induced cell death by modulating phosphorylation of JNK1-JNK2 (MAPK8 and MAPK9), p38 (MAPK11, MAPK12, MAPK13 and MAPK14) during oxidative stress. Plays a role in a staurosporine-induced caspase-independent apoptotic pathway by regulating the nuclear translocation of AIFM1 and ENDOG and the DNase activity associated with ENDOG. Phosphorylates STK38L on 'Thr-442' and stimulates its kinase activity. Regulates cellular migration with alteration of PTPN12 activity and PXN phosphorylation: phosphorylates PTPN12 and inhibits its activity and may regulate PXN phosphorylation through PTPN12. May act as a key regulator of axon regeneration in the optic nerve and radial nerve.
• Tissue specificityIsoform A is ubiquitous. Isoform B is expressed in brain with high expression in hippocampus and cerebral cortex.
• Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
  Contains 1 protein kinase domain.
• Post-translational
  modificationsProteolytically processed by caspases during apoptosis. Proteolytic cleavage results in kinase activation, nuclear translocation of the truncated form (MST3/N) and the induction of apoptosis.
  Isoform B is activated by phosphorylation by PKA. Oxidative stress induces phosphorylation. Activated by autophosphorylation at Thr-190 and phosphorylation at this site is essential for its function. Manganese, magnesium and cobalt-dependent autophosphorylation is mainly on threonine residues while zinc-dependent autophosphorylation is on both serine and threonine residues.
• Cellular localizationCytoplasm. Nucleus. Membrane. The truncated form (MST3/N) translocates to the nucleus. Co-localizes with STK38L in the membrane.

Target information above from: UniProt accession Q9Y6E0 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 8428 Human
  • Entrez Gene: 223255 Mouse
  • Entrez Gene: 361092 Rat
  • Omim: 604984 Human
  • SwissProt: Q9Y6E0 Human
  • SwissProt: Q99KH8 Mouse
  • SwissProt: B0LT89 Rat
  • Unigene: 508514 Human

  • Unigene: 390756 Mouse
  • Unigene: 442081 Mouse
  • Unigene: 472866 Mouse
  • Unigene: 105397 Rat

  see all

• Alternative names
  Mammalian STE20 like protein kinase 3 antibodyMammalian STE20-like protein kinase 3 antibodyMammalian STE20-like protein kinase 3 C-terminal antibody

  Mammalian STE20-like protein kinase 3 N-terminal antibodyMST-3 antibodyMST3/C antibodyMST3/N antibodyMST3B antibodyserine threonine kinase 24 antibodySerine/threonine kinase 24 (Ste20, yeast homolog) antibodySerine/threonine-protein kinase 24 12 kDa subunit antibodySTE20 homolog yeast antibodySTE20 like kinase MST3 antibodySTE20-like kinase MST3 antibodyStk24 antibodySTK24_HUMAN antibodySTK3 antibody

  see all

Anti-MST3 antibody [EP1468Y] images

• 

  Western blot - MST3 antibody [EP1468Y] (ab51137)
  Anti-MST3 antibody [EP1468Y] (ab51137) at 1/5000 dilution + HeLa cell lysate at 10 µg
  
  Secondary
  Goat anti-rabbit HRP labeled at 1/2000 dilution
  
  Predicted band size : 49 kDa
  Observed band size : 52 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MST3 antibody [EP1468Y] (ab51137)
  Ab51137 (1:100) staining human MST3 in human brain tissue by immunohistochemistry using paraffin embedded tissue.
• 

  Immunocytochemistry/ Immunofluorescence - MST3 antibody [EP1468Y] (ab51137)
  Immunofluorescent staining of HeLa cells using ab51137 (1:100).
• 

  Flow Cytometry-Anti-MST3 antibody [EP1468Y](ab51137)
  Overlay histogram showing HeLa cells stained with ab51137 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51137, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.