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Anti-SHP1 (phospho Y536) antibody (ab51171)

Abreviews (1)Q&A (3)Specific References (2)

Overview

  • Product nameAnti-SHP1 (phospho Y536) antibodySee all SHP1 primary antibodies ...
  • Description
    Rabbit polyclonal to SHP1 (phospho Y536)
  • Specificityab51171 detects endogenous levels of SHP1 only when phosphorylated at tyrosine 536.
  • Tested applicationsELISA, IHC-P, WB more details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic phosphopeptide derived from human SHP1 around the phosphorylation site of tyrosine 536 (S-E-YP-G-N).

  • Positive controlHuman breast carcinoma tissue and RAW264.7 cell extracts treated with EGF (200ng/ml, 5min).

Properties

Applications

Our Abpromise guarantee covers the use of ab51171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA ELISA: 1/10000.
IHC-P IHC-P: Use at an assay dependent dilution.
WB WB: 1/300 - 1/1000. Predicted molecular weight: 68 kDa.

Target

  • FunctionPlays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation.
  • Tissue specificityIsoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
  • Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
    Contains 2 SH2 domains.
    Contains 1 tyrosine-protein phosphatase domain.
  • Post-translational
    modifications    Phosphorylated on serine and tyrosine residues.
  • Cellular localizationCytoplasm. Nucleus. In neurons, translocates into the nucleus after treatment with angiotensin II.

    • Target information above from: UniProt accession P29350 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        70 kda SHP 1L protein antibody70 kda SHP1L protein antibody70Z-SHP antibody
        EC 3.1.3.48 antibodyHCP antibodyHCPH antibodyHematopoietic cell phosphatase antibodyHematopoietic cell protein tyrosine phosphatase antibodyHematopoietic cell protein-tyrosine phosphatase antibodyHPTP 1C antibodyHPTP1C antibodyProtein tyrosine phosphatase 1C antibodyProtein tyrosine phosphatase non receptor type 6 antibodyProtein tyrosine phosphatase SHP 1 antibodyProtein tyrosine phosphatase SHP1 antibodyProtein-tyrosine phosphatase 1C antibodyProtein-tyrosine phosphatase SHP-1 antibodyPTN6_HUMAN antibodyPTP 1C antibodyPTP-1C antibodyPTP1C antibodyPTPN 6 antibodyPTPN6 antibodySH PTP 1 antibodySH PTP1 antibodySH-PTP1 antibodySHP 1 antibodySHP 1L antibodySHP1 antibodySHP1L antibodySHPTP 1 antibodySHPTP1 antibodyTyrosine protein phosphatase non receptor type 6 antibodyTyrosine-protein phosphatase non-receptor type 6 antibody
      see all

    Anti-SHP1 (phospho Y536) antibody images

    • Immunohistochemistry (Paraffin-embedded sections) - SHP1 (phospho Y536) antibody (ab51171)
      Immunohistochemistry (Paraffin-embedded sections) - SHP1 (phospho Y536) antibody (ab51171)
      ab51171 at 1/50 dilution staining SHP1 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
    • Western blot - SHP1 (phospho Y536) antibody (ab51171)
      Western blot - SHP1 (phospho Y536) antibody (ab51171)


      Predicted band size : 68 kDa


      Western blot analysis of extracts from RAW264.7 cells treated with EGF (200ng/ml, 5 minutes)

    References for Anti-SHP1 (phospho Y536) antibody (ab51171)

    This product has been referenced in:
    • Ketroussi F  et al. Lymphocyte Cell-Cycle Inhibition by HLA-G Is Mediated by Phosphatase SHP-2 and Acts on the mTOR Pathway. PLoS One 6:e22776 (2011). WB ; Human . Read more (PubMed: 21887223) »
    • Shanmugarajan S  et al. Osteoclast inhibitory peptide-1 binding to the Fc gammaRIIB inhibits osteoclast differentiation. Endocrinology 151:4389-99 (2010). WB ; Mouse . Read more (PubMed: 20610564) »

    See all 2 Publications for this product

    Publishing research using ab51171 ? Please let us know so that we can cite the reference in this datasheet

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    Submit a Question for ab51171

    Displaying 1 - 3 of 3 results for Q&A

    10
    Votes: Highest First

    Thanks for your response. I am interested in trying ab51171 Anti-SHP1 (phospho Y536) antibody ab131500 Anti-SHP1 (phospho Y536) antibody first. In the website, these two products seem to be carrier protein free. Do they include any protein in...

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    Thank you foryour reply.

    The buffers for these product do not contain stabilizing proteins.



    Ab51171: Preservative: 0.02% Sodium Azide

    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chl...

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    On your website, there are 5 different clones of anti-phospho-SHP-1 antibodies. Has any one of them been tested for flow cytometry before? In my situation, even with AbTrial program, I still need to order all five of them and use a small amount of anti...

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    Thank you for your reply.

    Unfortunately none of these products has yet been tested in flow cytometry. While we do try and test as many products as we can in a large number of applications, it does seem as this group has not yet been though FC...

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    ab47282 and ab51171 give no signal in WB on human cells.

    Thank you for your phone call. As I mentioned, because these are phospho-specific antibodies, we recommend using BSA as a blocking agent instead of milk. If this does not help to improve your results, please let me knowyour original order or PO numberand ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"