Overview
- Product nameAnti-LC3B antibodySee all LC3B primary antibodies ...
- DescriptionRabbit polyclonal to LC3B
- Specificityab51520 is specific for the B isoform of LC3.
- Tested applicationsIHC - Wholemount, IHC-Fr, WB, ICC/IF more details
- Species reactivityReacts with: Mouse, Human
- Immunogen
Synthetic peptide made to the N terminal region of human LC3, isoform B protein.
- Positive controlWB: Treated U87-MG lysates. ICC/IF: Treated U87-MG cells. human malignant brain tumors, breast cancer, leukemia samples and murine cells
- General notes
ab51520 has been shown to work on human malignant brain tumors, breast cancer, leukemia samples, and murine cells.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.05% Sodium azide
Constituents: Tris glycine, 0.675% Glycine, 1.21% Tris, 0.87% Sodium chloride -
Concentration information loading... - PurityImmunogen affinity purified
- Primary antibody notes ab51520 has been shown to work on human malignant brain tumors, breast cancer, leukemia samples, and murine cells.
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab51520 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC - Wholemount | IHC - Wmt: Use at an assay dependent concentration. |
| IHC-Fr | IHC-Fr: 1/2000. |
| WB | WB: 1/3000. Predicted molecular weight: 15 kDa. Detects bands of ~16 kDa (LC3-II) and ~18 kDa (LC3-I). |
| ICC/IF | ICC/IF: 1/2000. |
Target
- FunctionProbably involved in formation of autophagosomal vacuoles (autophagosomes).
- Tissue specificityMost abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.
- Sequence similaritiesBelongs to the MAP1 LC3 family.
- Post-translational
modificationsThe precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II. - Cellular localizationCytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.
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Database links
- Entrez Gene: 81631 Human
- Entrez Gene: 67443 Mouse
- Omim: 609604 Human
- SwissProt: Q9GZQ8 Human
- SwissProt: Q9CQV6 Mouse
- Unigene: 356061 Human
- Unigene: 28357 Mouse
Target information above from: UniProt accession
Q9GZQ8
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- ATG8F antibodyAutophagy related protein LC3 B antibodyAutophagy related ubiquitin like modifier LC3 B antibody
- Autophagy-related protein LC3 B antibodyAutophagy-related ubiquitin-like modifier LC3 B antibodyLC3B antibodyMAP1 light chain 3 like protein 2 antibodyMAP1 light chain 3-like protein 2 antibodyMAP1A/1B light chain 3 B antibodyMAP1A/1BLC3 antibodyMAP1A/MAP1B LC3 B antibodyMAP1A/MAP1B light chain 3 B antibodyMAP1ALC3 antibodyMAP1LC3B a antibodyMAP1LC3B antibodyMicrotubule associated protein 1 light chain 3 beta antibodyMicrotubule associated proteins 1A/1B light chain 3B antibodyMicrotubule-associated protein 1 light chain 3 beta antibodyMicrotubule-associated proteins 1A/1B light chain 3B antibodyMLP3B_HUMAN antibody
see all
Anti-LC3B antibody images
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Western blot - LC3B antibody (ab51520)All lanes : Anti-LC3B antibody (ab51520) at 1/3000 dilution
Lane 1 : treated U87-MG (human glioblastoma astrocytoma) lysates with Arsenic Trioxide at 0 µM
Lane 2 : treated U87-MG (human glioblastoma astrocytoma) lysates with Arsenic Trioxide at 2 µM
Lane 3 : treated U87-MG (human glioblastoma astrocytoma) lysates with Arsenic Trioxide at 4 µM
Lane 4 : treated U87-MG (human glioblastoma astrocytoma) lysates with Arsenic Trioxide at 8 µM
Predicted band size : 15 kDa -
Immunocytochemistry/ Immunofluorescence - LC3B antibody (ab51520)Ab51520 at 1/2000 dilution staining treated U373-MG cells;
1) Control
2) C2-ceramide.
The nuclei were stained with DAPI. -
Immunocytochemistry/ Immunofluorescence - LC3B antibody (ab51520)Ab51520 at 1/2000 dilution staining treated U373-MG (human glioblastoma) cells
1) Control
2) Temozolomide -
Immunohistochemistry (Frozen sections) - LC3B antibody (ab51520)Ab51520 at 1/2000 dilution staining treated U87-MG cultured and subcutaneous tumors
1) Control
2) Temozolomide -
Immunohistochemistry (Frozen sections) - LC3B antibody (ab51520)Ab51520 at 1/2000 dilution staining gliobastoma multiform tissue
1) normal brain
2) gliobastoma multiform tissue
References for Anti-LC3B antibody (ab51520)
This product has been referenced in:
- Ma Y et al. Toll-like receptor (TLR) 2 and TLR4 differentially regulate doxorubicin induced cardiomyopathy in mice. PLoS One 7:e40763 (2012). WB ; Mouse . Read more (PubMed: 22808256) »
- Vandergaast R & Fredericksen BL West Nile Virus (WNV) Replication Is Independent of Autophagy in Mammalian Cells. PLoS One 7:e45800 (2012). WB . Read more (PubMed: 23029249) »
