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Overview

  • Product nameCaspase 10 protein (Active)See all Caspase 10 proteins and peptides ...
  • Protein descriptionActive Recombinant Human Caspase-10/a
  • Expression hostE. coli

    • Properties

  • Purity> 90 % by SDS-PAGE
  • Biological activitySPECIFIC ACTIVITY: 8000 units/mg One unit of the recombinant caspase-10/a is the enzyme activity that cleaves 1 nmol of the caspase substrate IETD-pNA (pNA: pnitroanaline) per hour at 37oC.
  • FormLyophilised:Reconstitute to 1 unit per µl in PBS containing 15% glycerol. Following reconstitution in PBS and 15% glycerol, the enzyme should be aliquoted and immediately stored at –70oC. Avoid multiple freeze/thaw cycles as activity might decrease.
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 15% Glycerol, PBS
  • Concentration information loading...
  • Additional notesThe lyophilized caspase-10 is stable for 1 year at –70oC.
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab52080 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    Functional Studies
  • Application notesFuncS: Active caspase10/a is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. Recommend using 1 unit/assay for analyzing caspase activity.

    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        ALPS2Apoptotic protease Mch-4CASP 10
        CASP-10CASP10CASPA_HUMANCaspase 10 apoptosis related cysteine peptidaseCaspase-10 subunit p12FADD like ICE2Fas associated death domain proteinFAS-associated death domain protein interleukin-1B-converting enzyme 2FLICE 2FLICE2ICE like apoptotic protease 4ICE-like apoptotic protease 4Interleukin 1B converting enzyme 2MCH 4
      see all
  • FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-
    -AMC and Asp-Glu-Val-Asp-
    -AMC.
    Isoform C is proteolytically inactive.
  • Tissue specificityDetectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon.
  • Involvement in diseaseDefects in CASP10 are the cause of autoimmune lymphoproliferative syndrome type 2A (ALPS2A) [MIM:603909]. ALPS2 is characterized by abnormal lymphocyte and dendritic cell homeostasis and immune regulatory defects.
    Defects in CASP10 are a cause of familial non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss.
    Defects in CASP10 are a cause of gastric cancer (GASC) [MIM:613659]. A malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease.
  • Sequence similaritiesBelongs to the peptidase C14A family.
    Contains 2 DED (death effector) domains.
  • Post-translational
    modifications    Cleavage by granzyme B and autocatalytic activity generate the two active subunits.
    Phosphorylated upon DNA damage, probably by ATM or ATR.

    • Target information above from: UniProt accession Q92851 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for Caspase 10 protein (Active) (ab52080)

    ab52080 has not yet been referenced specifically in any publications.

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