Anti-GM130 antibody [EP892Y] (ab52649)

Overview

• Product nameAnti-GM130 antibody [EP892Y]See all GM130 primary antibodies ...
• Description
  Rabbit monoclonal [EP892Y] to GM130
• SpecificityMouse and rat cell lines pc12, 3t3, raw 264.7 were tested positive in WB. However, brain, kidney, spleen and heart were negative from the two species.
• Tested applicationsICC/IF, IHC-P, IHC-Fr, WB, IP more details
• Species reactivity
  Reacts with: Mouse, Rat, Cow, Dog, Human, African Green Monkey
  
• Immunogen

  A synthetic peptide corresponding to residues near the N-term of human GM130.

• Positive controlHela cell lysate and human liver tissue.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• Concentration information loading...
• Clonality Monoclonal
• Clone numberEP892Y
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Protein Trafficking
  • Golgi Proteins

  • Tags & Cell Markers
  • Subcellular Markers
  • Organelles
  • Golgi

Applications

Target

• FunctionGolgi auto-antigen; probably involved in maintaining cis-Golgi structure.
• Sequence similaritiesBelongs to the GOLGA2 family.
• DomainExtended rod-like protein with coiled-coil domains.
• Cellular localizationGolgi apparatus > Golgi stack membrane.

Target information above from: UniProt accession Q08379 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2801 Human
  • Entrez Gene: 99412 Mouse
  • Entrez Gene: 64528 Rat
  • Omim: 602580 Human
  • SwissProt: Q08379 Human
  • SwissProt: Q921M4 Mouse
  • SwissProt: Q62839 Rat
  • Unigene: 155827 Human

  • Unigene: 106376 Mouse
  • Unigene: 6165 Rat

  see all

• Alternative names
  130 kDa cis Golgi matrix protein antibody130 kDa cis-Golgi matrix protein antibodyCis golgi matrix protein GM130 antibody

  GM130 antibodyGM130 autoantigen antibodyGOGA2_HUMAN antibodyGOLGA 2 antibodyGOLGA2 antibodyGolgi autoantigen antibodyGolgi autoantigen golgin subfamily a 2 antibodyGolgi matrix protein GM130 antibodyGolgin 95 antibodygolgin A2 antibodyGolgin subfamily a 2 antibodyGolgin subfamily A member 2 antibodyGolgin-95 antibodyMGC20672 antibodySY11 protein antibody

  see all

Anti-GM130 antibody [EP892Y] images

• 

  Western blot - GM130 antibody [EP892Y] (ab52649)
  Anti-GM130 antibody [EP892Y] (ab52649) at 1/200000 dilution + 10 µg of HeLa cell lysate
  
  Secondary
  Goat anti-rabbit HRP labeled at 1/2000 dilution
  
  Predicted band size : 112 kDa
  
• 

  Immunohistochemistry (Paraffin-embedded sections) - GM130 antibody [EP892Y] (ab52649)
  ab52649 (1/500) staining GM130 in paraffin-embedded Human liver tissue sections.
• 

  Immunocytochemistry/ Immunofluorescence - GM130 antibody [EP892Y] (ab52649)This image is courtesy of an Abreview submitted by Dr Vladimir Milenkovic
  ab52649 staining GM130 in human ARPE-19 cells by ICC/IF (immunocytochemistry/immunofluorescence). Cells were formaldehyde fixed, permeabilized by 0.5% TX-100 and blocked with 5% serum for 20 minutes at 25°C. The sample was incubated with the primary antibody (1/500 in 1% goat serum, 0.1%TX100, 1 x PBS) for 16 hours at 4°C. An Alexa Fluor^®488-conjugated Goat anti-rabbit polyclonal (1/500) was used as the secondary.

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - GM130 antibody [EP892Y] (ab52649)This image is courtesy of an Abreview submitted by JL Balligand
  ab52649 staining GM130 in Bovine brain microvascular endothelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% saponin and blocked with 5% BSA for 90 minutes at 37°C. Samples were incubated with primary antibody (1/100 in 0.1% saponin + 1% BSA ) for 18 hours at 4°C. An undiluted Alexa Fluor^®568-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - GM130 antibody [EP892Y] (ab52649)
  ICC/IF image of ab52946 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52946, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
• 

  Immunocytochemistry/ Immunofluorescence - GM130 antibody [EP892Y] (ab52649)
  ICC/IF image of ab52649 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52649, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.