Anti-PML Protein antibody (ab53773)

Overview

• Product nameAnti-PML Protein antibodySee all PML Protein primary antibodies ...
• Description
  Rabbit polyclonal to PML Protein
• SpecificityThis antibody detects endogenous levels of total PML protein.
• Tested applicationsWB, ELISA, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Synthetic peptide derived from human PML protein

• Positive controlExtracts from A549 cells

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Hypoxia

  • Cancer
  • Cancer Metabolism
  • Response to hypoxia

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • Zinc Finger

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Transcription Factors

Applications

Target

• FunctionKey component of PML nuclear bodies that regulate a large number of cellular processes by facilitating post-translational modification of target proteins, promoting protein-protein contacts, or by sequestering proteins. Functions as tumor suppressor. Required for normal, caspase-dependent apoptosis in response to DNA damage, FAS, TNF, or interferons. Plays a role in transcription regulation, DNA damage response, DNA repair and chromatin organization. Plays a role in processes regulated by retinoic acid, regulation of cell division, terminal differentiation of myeloid precursor cells and differentiation of neural progenitor cells. Required for normal immunity to microbial infections. Plays a role in antiviral response. In the cytoplasm, plays a role in TGFB1-dependent processes. Regulates p53/TP53 levels by inhibiting its ubiquitination and proteasomal degradation. Regulates activation of p53/TP53 via phosphorylation at 'Ser-20'. Sequesters MDM2 in the nucleolus after DNA damage, and thereby inhibits ubiquitination and degradation of p53/TP53. Regulates translation of HIF1A by sequestering MTOR, and thereby plays a role in neoangiogenesis and tumor vascularization. Regulates RB1 phosphorylation and activity. Required for normal development of the brain cortex during embryogenesis. Can sequester herpes virus and varicella virus proteins inside PML bodies, and thereby inhibit the formation of infectious viral particles. Regulates phosphorylation of ITPR3 and plays a role in the regulation of calcium homeostasis at the endoplasmic reticulum (By similarity). Regulates transcription activity of ELF4. Inhibits specifically the activity of the tetrameric form of PKM2. Together with SATB1, involved in local chromatin-loop remodeling and gene expression regulation at the MHC-I locus. Regulates PTEN compartmentalization through the inhibition of USP7-mediated deubiquitinylation.
• Involvement in diseaseNote=A chromosomal aberration involving PML may be a cause of acute promyelocytic leukemia (APL). Translocation t(15;17)(q21;q21) with RARA. The PML breakpoints (type A and type B) lie on either side of an alternatively spliced exon.
• Sequence similaritiesContains 2 B box-type zinc fingers.
  Contains 1 RING-type zinc finger.
• DomainInteracts with PKM2 via its coiled-coil domain.
  Binds arsenic via the RING-type zinc finger.
• Post-translational
  modificationsUbiquitinated; mediated by RNF4, SIAH1 or SIAH2 and leading to subsequent proteasomal degradation. 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination by RNF4 is polysumoylation-dependent.
  Undergoes 'Lys-11'-linked sumoylation. Sumoylation on all three sites is required for nuclear body formation. Sumoylation on Lys-160 is a prerequisite for sumoylation on Lys-65. The PML-RARA fusion protein requires the coiled-coil domain for sumoylation. Desumoylated by SENP2 and SENP6. Arsenic induces PML and PML-RARA oncogenic fusion proteins polysumoylation and their subsequent RNF4-dependent ubiquitination and proteasomal degradation, and is used as treatment in acute promyelocytic leukemia (APL).
  Phosphorylated in response to DNA damage, probably by ATR.
  Acetylation may promote sumoylation and enhance induction of apoptosis.
• Cellular localizationNucleus > nucleoplasm. Cytoplasm. Nucleus > PML body. Nucleus > nucleolus. Endoplasmic reticulum membrane. Early endosome membrane. Sumoylated forms localize to the PML nuclear bodies. The B1 box and the RING finger are also required for this nuclear localization. Isoforms lacking a nuclear localization signal are cytoplasmic. Detected in the nucleolus after DNA damage. Sequestered in the cytoplasm by interaction with rabies virus phosphoprotein.

Target information above from: UniProt accession P29590 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5371 Human
  • Entrez Gene: 18854 Mouse
  • Omim: 102578 Human
  • SwissProt: P29590 Human
  • SwissProt: Q60953 Mouse
  • Unigene: 526464 Human
  • Unigene: 392123 Mouse

• Alternative names
  MYL antibodyPML antibodyPML_HUMAN antibody

  PP8675 antibodyProbable transcription factor PML antibodyPromyelocytic leukemia antibodyPromyelocytic leukemia inducer of antibodyPromyelocytic leukemia protein antibodyProtein PML antibodyRING finger protein 71 antibodyRNF 71 antibodyRNF71 antibodyTRIM 19 antibodyTRIM19 antibodyTripartite motif containing protein 19 antibodyTripartite motif protein TRIM19 antibodyTripartite motif-containing protein 19 antibody

  see all

Anti-PML Protein antibody images

• 

  Western blot - PML Protein antibody (ab53773)
  All lanes : Anti-PML Protein antibody (ab53773) at 1/500 dilution
  
  Lane 1 : Extracts from A549 cells
  Lane 2 : Extracts from A549 cells, plus immunising peptide
  
  
  Predicted band size : 69 kDa
  Observed band size : 100 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunocytochemistry/ Immunofluorescence - PML Protein antibody (ab53773)This image is courtesy of an Abreview submitted by Dr Kirk McManus
  ab53773 (1/200) staining PML protein in Hela cells (green). Cells were fixed with methanol, permeabilised with triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PML Protein antibody (ab53773)
  Ab53773 staining Human normal colon. Staining is localized to the nucleus.
  Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
  Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.