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Overview

  • Product nameVEGF Receptor 1 protein (Fc Chimera)See all VEGF Receptor 1 proteins and peptides ...
  • Protein descriptionRecombinant fragment: human soluble Vascular Endothelial Growth Factor Receptor 1, fused with the Fc part of human IgG 1. Recombinant sVEGFR1/Fc is a disulfide-linked homodimeric protein, containing all 7 extracellular domains, M.Wt ~130 kDa (monomer), produced in insect cells.
  • Expression hostInsect cells

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Purification notesab54258 purity is > 95%, by SDS-PAGE and visualised by silver stain. Endotoxin level: < 0.1 ng per ug of sVEGFR-1
  • FormLyophilised:Reconstitute in PBS or culture medium to a concentration not lower than 50 µg/ml.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab54258 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    Functional Studies
  • Application notesSDS-PAGE: Use at an assay dependent dilution.
    Biological Activity: The activity of sVEGFR 1/Fc was determined by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells. The ED 50 for this effect is typically 10-30 ng/ml.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        EC 2.7.10.1FLTFlt 1
        Flt-1Flt1Fms like tyrosine kinase 1Fms related tyrosine kinase 1Fms related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)Fms related tyrosine kinase 1 vascular endothelial growth factor/vascular permeability factor receptorFms-like tyrosine kinase 1FRTSoluble VEGF receptor 1 14Soluble VEGFR1 variant 2Soluble VEGFR1 variant 21Tyrosine protein kinase FRTTyrosine protein kinase receptor FLTTyrosine-protein kinase FRTTyrosine-protein kinase receptor FLTVascular endothelial growth factor receptor 1Vascular endothelial growth factor receptor 1 PrecursorVascular endothelial growth factor vascular permeability factor receptorVascular permeability factor receptorVascular permeability factor receptor 1VEGFR 1VEGFR-1VEGFR1VGFR1_HUMAN
      see all
  • FunctionReceptor for VEGF, VEGFB and PGF. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Isoform SFlt1 may have an inhibitory role in angiogenesis.
  • Tissue specificityMostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. It is not expressed in tumor cell lines. Isoform sFlt1 is strongly expressed in placenta.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Cellular localizationSecreted and Cell membrane.

    • Target information above from: UniProt accession P17948 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for VEGF Receptor 1 protein (Fc Chimera) (ab54258)

    This product has been referenced in:
    • Kaczmarek MM  et al. Assessment of VEGF-receptor system expression in the porcine endometrial stromal cells in response to insulin-like growth factor-I, relaxin, oxytocin and prostaglandin E2. Mol Cell Endocrinol 291:33-41 (2008). Read more (PubMed: 18562087) »

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