Anti-PDGF Receptor alpha (phospho Y754) antibody (ab5460)

Overview

• Product nameAnti-PDGF Receptor alpha (phospho Y754) antibodySee all PDGF Receptor alpha primary antibodies ...
• Description
  Rabbit polyclonal to PDGF Receptor alpha (phospho Y754)
• SpecificityPDGFR beta (50%) has not been tested, but is not expected to react.
• Tested applicationsICC/IF, IHC-P, WB more details
• Species reactivity
  Reacts with: Mouse, Human
  Predicted to work with: Rat, Xenopus laevis
• Immunogen

  Synthetic phospho peptide (Human) containing Tyrosine 754.

• Positive controlNIH3T3 cells +/- PDGF.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.05% Sodium Azide
  Constituents: PBS, 1mg/ml BSA (IgG, protease free). pH 7.3
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesThe antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PDGFR alpha. The final product is generated by affinity chromatography using a PDGFR alpha derived peptide phosphorylated at tyrosine 754.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Oncoproteins/suppressors
  • Oncoproteins
  • Growth factor receptors

  • Cancer
  • Growth factors
  • PDGF

  • Signal Transduction
  • Growth Factors/Hormones
  • PDGF

  • Signal Transduction
  • Protein Phosphorylation
  • Tyrosine Kinases
  • Receptor Tyrosine Kinases

  • Cardiovascular
  • Atherosclerosis
  • Vascular Inflammation
  • Inflammatory mediators

  • Cardiovascular
  • Angiogenesis
  • Growth Factors
  • Other

Applications

Target

• FunctionReceptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity.
• Tissue specificityExpressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue.
• Involvement in diseaseNote=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA).
• Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
  Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  Contains 1 protein kinase domain.
• Cellular localizationMembrane.

Target information above from: UniProt accession P16234 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5156 Human
  • Entrez Gene: 18595 Mouse
  • Entrez Gene: 25267 Rat
  • Omim: 173490 Human
  • SwissProt: P16234 Human
  • SwissProt: P26618 Mouse
  • SwissProt: P20786 Rat
  • SwissProt: P26619 Xenopus laevis

  • Unigene: 74615 Human
  • Unigene: 221403 Mouse
  • Unigene: 55127 Rat
  • Unigene: 20029 Xenopus laevis

  see all

• Alternative names
  Alpha platelet derived growth factor receptor antibodyAlpha-type platelet-derived growth factor receptor antibodyCD 140a antibody

  CD140 antigen-like family member A antibodyCD140a antibodyCD140a antigen antibodyMGC74795 antibodyPDGF alpha chain antibodyPDGF R alpha antibodyPDGF-R-alpha antibodyPDGFR 2 antibodyPDGFR A antibodyPDGFR alpha antibodyPDGFR2 antibodyPDGFRA antibodyPDGFRA/BCR fusion antibodyPGFRA_HUMAN antibodyPlatelet derived growth factor receptor 2 antibodyPlatelet derived growth factor receptor alpha antibodyPlatelet derived growth factor receptor alpha polypeptide antibodyPlatelet derived growth factor receptor antibodyRearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein antibodyRHEPDGFRA antibody

  see all

Anti-PDGF Receptor alpha (phospho Y754) antibody images

• 

  Western blot - Anti-PDGF Receptor alpha (phospho Y754) antibody (ab5460)
  Peptide Competition: Extracts prepared from NIH3T3 cells left unstimulated (1) and stimulated with PDGF (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.50 µg/mL ab5460 antibody for two hours at room temperature in a 1% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphotyrosine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab5460 blocks the antibody signal, thereby demonstrating the specificity of the antibody.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDGF Receptor alpha (phospho Y754) antibody (ab5460)Image courtesy of an anonymous Abreview.
  ab5460 staining PDGF Receptor alpha (phospho Y754) in murine brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using citric acid pH 6.1. Samples were then permeabilized using 0.3% H₂O₂, then blocked with 0.5% BSA for 20 minutes at room temperature, followed by incubation with the primary antibody at a 1/50 dilution for 1 hour. An undiluted HRP-conjugated Goat anti-rabbit/mouse polyclonal was used as secondary antibody. Nuclei staining with Haematoxillin (blue) and PDGF Receptor alpha staining with DAB (brown). PDGF Receptor alpha staining in the membrane of astrocytes.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDGF Receptor alpha (phospho Y754) antibody (ab5460)Image courtesy of an anonymous Abreview.
  ab5460 staining PDGF Receptor alpha (phospho Y754) in human glioblastoma multiforme brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using citric acid pH 6.1. Samples were then permeabilized using 0.3% H2O2, then blocked with 0.5% BSA for 20 minutes at room temperature, followed by incubation with the primary antibody at a 1/50 dilution for 1 hour. An undiluted HRP-conjugated Goat anti-rabbit/mouse polyclonal was used as secondary antibody. Nuclei staining with Haematoxillin (blue) and PDGF Receptor alpha staining with DAB (brown). PDGF Receptor alpha staining in the membrane of astrocytes.

  See Abreview

Protocols

• Peptide Competition Experiment
• Western blotting protocol