Overview
- Product nameAnti-eNOS antibodySee all eNOS primary antibodies ...
- DescriptionRabbit polyclonal to eNOS
- SpecificityThis antibody does not detect Human inducible NOS or rat brain nNOS. ab5589 has been successfully used in Western blot and immunohistochemistry (paraffin and frozen) procedures. By Western blot, this antibody detects an ~140 kDa protein representing eNOS from Human endothelial cells. By Western blot ab5589 also detects eNOS in rat lung, brain, and canine cardiac microsomes. Immunohistochemical staining of eNOS in Human vascular endothelial cells with ab5589 results in prominent staining of the epithelial cells.
- Tested applicationsIHC-P, IHC-Fr, WB more details
- Species reactivityReacts with: Mouse, Rat, Guinea pig, Cow, Dog, Human, Pig
- Immunogen
Synthetic peptide: SLQERQLRGAVPWAFD, corresponding to amino acids 1179-1194 of Human NOS (eNOS).
- Positive controlIHC: Human endothelial cells. WB:canine cardiac microsomes and rat or human lung lysate.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.05% Sodium Azide
Constituents: PBS, 1mg/ml BSA -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab5589 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-P | IHC-P: 1/100. Antigen retrieval is not essential but may optimise staining. |
| IHC-Fr | IHC-Fr: 1/100. |
| WB | WB: 1/1000. Predicted molecular weight: 133 kDa. By Western blot ab5589 also detects eNOS in canine cardiac microsomes and rat lung. |
Target
- FunctionProduces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
- Tissue specificityPlatelets, placenta, liver and kidney.
- Sequence similaritiesBelongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain. - Cellular localizationCell membrane. Membrane > caveola. Cytoplasm > cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle and which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
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Database links
- Entrez Gene: 287024 Cow
- Entrez Gene: 403784 Dog
- Entrez Gene: 4846 Human
- Entrez Gene: 18127 Mouse
- Entrez Gene: 397557 Pig
- Entrez Gene: 24600 Rat
- Omim: 163729 Human
- SwissProt: P29473 Cow
- SwissProt: Q9TUX8 Dog
- SwissProt: P97270 Guinea pig
- SwissProt: P29474 Human
- SwissProt: P70313 Mouse
- SwissProt: Q28969 Pig
- SwissProt: Q62600 Rat
- Unigene: 4662 Cow
- Unigene: 108 Dog
- Unigene: 647092 Human
- Unigene: 258415 Mouse
- Unigene: 16364 Pig
- Unigene: 44265 Rat
see all
Target information above from: UniProt accession
P29474
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- cNOS antibodyConstitutive NOS antibodyEC NOS antibody
- EC-NOS antibodyecNOS antibodyEndothelial nitric oxidase synthase antibodyEndothelial nitric oxide synthase antibodyEndothelial nitric oxide synthase 3 antibodyEndothelial NOS antibodyeNOS antibodyNitric oxide synthase 3 (endothelial cell) antibodyNitric oxide synthase 3 antibodyNitric oxide synthase 3 antibodyNitric oxide synthase 3 endothelial cell antibodyNitric oxide synthase endothelial antibodyNitric oxide synthase, endothelial antibodyNOS 3 antibodyNOS III antibodyNOS type III antibodyNOS3 antibodyNOS3_HUMAN antibodyNOSIII antibody
see all
Anti-eNOS antibody images
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- eNOS antibody (ab5589)Immunolocalization of eNOS in human lung tissue using ab5589.
References for Anti-eNOS antibody (ab5589)
This product has been referenced in:
- Qi Y et al. Inhaled NO contributes to lung repair in piglets with acute respiratory distress syndrome via increasing circulating endothelial progenitor cells. PLoS One 7:e33859 (2012). IHC-P ; Pig . Read more (PubMed: 22448277) »
- Konior A et al. Seasonal superoxide overproduction and endothelial activation in guinea-pig heart; seasonal oxidative stress in rats and humans. J Mol Cell Cardiol 50:686-94 (2011). WB ; Rat, Guinea pig . Read more (PubMed: 21112335) »
