Anti-c-Myc antibody [9E11] - ChIP Grade (ab56)

Overview

• Product nameAnti-c-Myc antibody [9E11] - ChIP GradeSee all c-Myc primary antibodies ...
• Description
  Mouse monoclonal [9E11] to c-Myc - ChIP Grade
• Conjugation notes-
• Tested applicationsFlow Cyt, ChIP, WB, IP, IHC-P, IHC-Fr more details
• Species reactivity
  Reacts with: Chicken, Human, Saccharomyces cerevisiae
  
• Immunogen

  Synthetic peptide: AEEQKLISEEDL conjugated to KLH, corresponding to amino acids 408-420 of Human c-Myc.

• Positive controlHL-60 cells or breast tumors. Flow Cyt: This antibody gave a positive signal in paraformaldehyde fixed/Tween permeabilised HL60 cells.
• General notesWe can conjugate this antibody to FITC for you (please see ab150238 for details).

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPhosphate buffered saline
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Monoclonal
• Clone number9E11
• MyelomaSp2
• IsotypeIgG2a
• Light chain typekappa
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • TGF beta
  • Nuclear

  • Cancer
  • Oncoproteins/suppressors
  • Oncoproteins
  • Transcription factors

  • Cancer
  • Tumor biomarkers
  • Oncoproteins

  • Cancer
  • Cell cycle
  • Cell differentiation

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Transcription Factors

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • HLH / Leucine Zipper
  • HLH / Leucine Zipper

  • Epigenetics and Nuclear Signaling
  • ChIP'ing antibodies
  • ChIP'ing antibodies

Applications

Target

• FunctionParticipates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
• Involvement in diseaseNote=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
  Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
  Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci.
• Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
• Post-translational
  modificationsPhosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
  Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex.
• Cellular localizationNucleus > nucleoplasm. Nucleus > nucleolus.

Target information above from: UniProt accession P01106 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 4609 Human
  • Omim: 190080 Human
  • SwissProt: P01109 Chicken
  • SwissProt: P01106 Human
  • Unigene: 202453 Human

• Alternative names
  AU016757 antibodyAvian myelocytomatosis viral oncogene homolog antibodybHLHe39 antibody

  c Myc antibodyCellular myelocytomatosis oncogene antibodyClass E basic helix-loop-helix protein 39 antibodyMGC105490 antibodyMRTL antibodyMyc antibodyMyc protein antibodyMyc proto oncogene protein antibodyMyc proto-oncogene protein antibodyMyc-related translation/localization regulatory factor antibodyMYC_HUMAN antibodyMyc2 antibodymyca antibodyMyelocytomatosis oncogene a antibodyMyelocytomatosis oncogene antibodyNiard antibodyNird antibodyOncogene Myc antibodyOTTHUMP00000158589 antibodyOTTHUMP00000227763 antibodyProto-oncogene c-Myc antibodyProtooncogene homologous to myelocytomatosis virus antibodyRNCMYC antibodyTranscription factor p64 antibodyTranscriptional regulator Myc-A antibodyv myc avian myelocytomatosis viral oncogene homolog antibodyv myc myelocytomatosis viral oncogene homolog (avian) antibodyV-Myc avian myelocytomatosis viral oncogene homolog antibodyv-myc myelocytomatosis viral oncogene homolog (avian) antibodyzc-myc antibody

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Anti-c-Myc antibody [9E11] - ChIP Grade images

• 

  Immunoprecipitation - Anti-c-Myc antibody [9E11] - ChIP Grade (ab56)

  c-Myc was immunoprecipitated using 0.5mg CHO overexpressing Stra8 whole cell lysate, 5µg of Mouse monoclonal to c-Myc and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

  The antibody was incubated under agitation with Protein G beads for 10min, CHO overexpressing Stra8 whole cell lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

  Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab56.

  Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.

  Band: 49KDa; c-Myc

• 

  Flow Cytometry - c-Myc antibody [9E11] - ChIP Grade (ab56)
  Overlay histogram showing HL60 cells stained with ab56 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.
  
  
• 

  Western blot - c-Myc antibody [9E11] - ChIP Grade (ab56)Image courtesy of an anonymous Abreview.
  Anti-c-Myc antibody [9E11] - ChIP Grade (ab56) at 1/1000 dilution + whole cell lysate prepared from huh-7 cells at 15 µg
  
  Secondary
  Anti-mouse IgG conjugated to HRP at 1/10000 dilution
  
  Predicted band size : 49 kDa
  

  Image courtesy of an anonymous Abreview.

• 

  Western blot
  Anti-c-Myc antibody [9E11] - ChIP Grade (ab56) at 1/500 dilution + c-Myc protein (Human) (ab84132) at 0.01 µg
  
  Secondary
  Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Exposure time : 4 minutes