Overview
- Product nameAnti-CEACAM6 antibodySee all CEACAM6 primary antibodies ...
- DescriptionRabbit polyclonal to CEACAM6
- Tested applicationsIHC-Fr, WB, IHC-P more details
- Species reactivityReacts with: Human
- Immunogen
A region within synthetic peptide: IQNPASANRS DPVTLNVLYG PDGPTISPSK ANYRPGENLN LSCHAASNPP, corresponding to internal sequence amino acids 217-266 of Human CEACAM6
- Positive controlJurkat cell lysate and human lung tissue.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... - PurityProtein A purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab56234 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-Fr | |
| WB | |
| IHC-P |
IHC-Fr: Use at an assay dependent dilution (PMID 20220088).
WB: Use at a concentration of 1.25 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 37 kDa).
ELISA titre using peptide based assay: 1/312500.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- Sequence similaritiesBelongs to the immunoglobulin superfamily. CEA family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. - Cellular localizationCell membrane.
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Database links
- Entrez Gene: 4680 Human
- Omim: 163980 Human
- SwissProt: P40199 Human
- Unigene: 466814 Human
Target information above from: UniProt accession
P40199
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Carcinoembryonic antigen related cell adhesion molecule 6 antibodyCarcinoembryonic antigen related cell adhesion molecule 6 (non specific cross reacting antigen) antibodyCarcinoembryonic antigen-related cell adhesion molecule 6 antibody
- CD 66c antibodyCD66c antibodyCD66c antigen antibodyCEA LIKE PROTEIN antibodyCEACAM 6 antibodyCEACAM6 antibodyCEAL antibodyCEAM6_HUMAN antibodyMGC93832 antibodyNCA antibodyNon specific cross reacting antigen antibodyNon-specific crossreacting antigen antibodyNormal cross reacting antigen antibodyNormal cross-reacting antigen antibody
see all
Anti-CEACAM6 antibody images
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Western blot - CEACAM6 antibody (ab56234)All lanes : Anti-CEACAM6 antibody (ab56234) at 1.25 µg/ml
Lane 1 : Molecular weight markers
Lane 2 : Jurkat cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 37 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?) -
Immunohistochemistry (Paraffin-embedded sections) - CEACAM6 antibody (ab56234)ab56234 at 4µg/ml, staining Human CEACAM6 in human lung tissue by immunohistochemistry on paraffin embedded tissue. The arrows indicate positively stained alveolar cells. Magnification 400X. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-CEACAM6 antibody(ab56234)Immunohistochemistry of Human Lung lysate tissue at an antibody concentration of 4-8µg/ml using anti-CEACAM6 antibody (ab56234)
References for Anti-CEACAM6 antibody (ab56234)
This product has been referenced in:
- Allen CE et al. Cell-specific gene expression in Langerhans cell histiocytosis lesions reveals a distinct profile compared with epidermal Langerhans cells. J Immunol 184:4557-67 (2010). IHC-Fr ; Human . Read more (PubMed: 20220088) »
