Anti-alpha smooth muscle Actin antibody (ab5694)
Overview
- Product nameAnti-alpha smooth muscle Actin antibodySee all alpha smooth muscle Actin primary antibodies ...
- DescriptionRabbit polyclonal to alpha smooth muscle Actin
- SpecificityThis antibody stains smooth muscle cells in vessel walls, gut wall, and myometrium. Myoepithelial cells in breast and salivary gland are also stained. It reacts with tumors arising from smooth muscles and myoepithelial cells. The other actins, such as beta- and gamma-cytoplasmic, striated muscle, and myocardium are not stained by this antibody.
- Tested applicationsIHC-FoFr, ICC, ICC/IF, WB, ELISA, IHC-P, IHC-Fr more details
- Species reactivityReacts with: Mouse, Rat, Chicken, Guinea pig, Cow, Dog, Human, Pig
- Immunogen
Synthetic peptide (Human) corresponding to N-terminus of smooth muscle alpha actin.
- General notes
Actins are highly conserved proteins expressed in all eucaryotic cells. Actin filaments form part of the cytoskeleton and play essential roles in regulating cell shape and movement. Six distinct actin isotypes have been identified in mammalian cells. Each is encoded by a separated gene and is expressed in a developmentally regulated and tissue-specific manner, alpha and beta cytoplasmic actins are expressed in a wide variety of cells; whereas, expression of alpha skeletal, alpha cardiac, alpha vascular, and gamma enteric actins are more restricted to specialized muscle cell type. Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Further more, expression of smooth muscle alpha actin is regulated by hormones, cell proliferation , and altered by pathological conditions including oncogenic transformation and atherosclerosis.
Properties
- FormLiquid
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Primary antibody notes Actins are highly conserved proteins expressed in all eucaryotic cells. Actin filaments form part of the cytoskeleton and play essential roles in regulating cell shape and movement. Six distinct actin isotypes have been identified in mammalian cells. Each is encoded by a separated gene and is expressed in a developmentally regulated and tissue-specific manner, alpha and beta cytoplasmic actins are expressed in a wide variety of cells; whereas, expression of alpha skeletal, alpha cardiac, alpha vascular, and gamma enteric actins are more restricted to specialized muscle cell type. Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Further more, expression of smooth muscle alpha actin is regulated by hormones, cell proliferation , and altered by pathological conditions including oncogenic transformation and atherosclerosis.
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab5694 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-FoFr | IHC-FoFr: Use at an assay dependent dilution. |
| ICC | ICC: Use at an assay dependent concentration. |
| ICC/IF | ICC/IF: 1/100. |
| WB | WB: Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 42 kDa. |
| ELISA | ELISA: Use a concentration of 0.1 - 1 µg/ml. |
| IHC-P | IHC-P: 1/100 - 1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
| IHC-Fr | IHC-Fr: 1/200. PubMed: 18559614Fix with acetone. |
Target
- FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
- Involvement in diseaseDefects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
- Sequence similaritiesBelongs to the actin family.
- Cellular localizationCytoplasm > cytoskeleton.
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Database links
- Entrez Gene: 423787 Chicken
- Entrez Gene: 515610 Cow
- Entrez Gene: 59 Human
- Entrez Gene: 11475 Mouse
- Entrez Gene: 733615 Pig
- Entrez Gene: 81633 Rat
- Omim: 102620 Human
- SwissProt: P08023 Chicken
- SwissProt: P62739 Cow
- SwissProt: P62736 Human
- SwissProt: P62737 Mouse
- SwissProt: P62738 Rat
- Unigene: 500483 Human
- Unigene: 213025 Mouse
- Unigene: 195319 Rat
- Unigene: 3114 Rat
see all
Target information above from: UniProt accession
P62736
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- AAT6 antibodyACTA_HUMAN antibodyACTA2 antibody
- Actin alpha 2 smooth muscle aorta antibodyActin aortic smooth muscle antibodyActin, aortic smooth muscle antibodyACTSA antibodyACTVS antibodyAlpha 2 actin antibodyAlpha actin 2 antibodyAlpha cardiac actin antibodyAlpha-actin 2 antibodyAlpha-actin-2 antibodyCell growth inhibiting gene 46 protein antibodyCell growth-inhibiting gene 46 protein antibodyGIG46 antibodyGrowth inhibiting gene 46 antibodyMYMY5 antibody
see all
Anti-alpha smooth muscle Actin antibody images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab5694)Human Leiomyoma stained with ab5694. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab5694)This picture shows formalin-fixed, paraffin embedded mouse intestine and mesentery, the optimal dilution is 1:1600 to 1:3200, incubation overnight at 4oC, counterstained with Hematoxylin.
This image was kindly supplied as part of the review by JQ Zhang.
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Western blot - alpha smooth muscle Actin antibody (ab5694)All lanes : Anti-alpha smooth muscle Actin antibody (ab5694) at 1 µg/ml
Lane 1 : HeLa Nuclear
Lane 2 : HeLa whole cell
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : HEK293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Alexa Fluor anti-rabbit at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 42 kDa
Observed band size : 42 kDa
Additional bands at : 30 kDa,35 kDa,37 kDa,50 kDa,75 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Frozen sections) - alpha smooth muscle Actin antibody - Smooth Muscle Cell Marker (ab5694)Ab5694 positively staining smooth muscle cells in blood vessels and myoepithelial cells in the frozen tissue of cancerous human mammary gland (pink) at 1/100 dilution. Secondary: CY5 conjugated goat anti rabbit (1/100). Co immunostaining of glandular cell cytokeratin can be seen stained by FITC (green). Auto fluorescent erythrocytes that are present within blood vessels are shown (red), whilst the DAPI counter stain may clearly be seen staining nuclei (blue).
This image is courtesy of an Abreview submitted by on 22 August 2005. We do not have any further information relating to this image. -
Western blot - alpha smooth muscle Actin antibody - Smooth Muscle Cell Marker (ab5694)All lanes : Anti-alpha smooth muscle Actin antibody (ab5694) at 1/500 dilution
Lane 1 : Rat2 myofibroblasts (untreated before treatment-0 days)
Lane 2 : Rat2 myofibroblasts (untreated for 5 days)
Lane 3 : Rat2 myofibroblasts (treated with 1ng/mL TGF beta)
Lane 4 : Rat2 myofibroblasts (treated with 10ng/mL TGF beta)
Lane 5 : Positive control (NIH3T3)
Lane 6 : Negative control (MDA-MB-469 breast carcinoma cells)
Lysates/proteins at 10 µg per lane.
Secondary
Donkey anti rabbit (HRP) at 1/2500 dilution
Performed under reducing conditions.
Predicted band size : 42 kDa
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Immunocytochemistry/ Immunofluorescence - alpha smooth muscle Actin antibody (ab5694)This image is courtesy of an Abreview submitted by Dr Jianyuan Chaiab5694 at 1/500 staining rat myofibroblast cells by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed and blocked with 5% serum prior to incubation with the antibody for 2 hours. A FITC conjugated goat anti-rabbit IgG was used as the secondary. Nuclei were counterstained with propidium iodide.
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Immunocytochemistry/ Immunofluorescence - alpha smooth muscle Actin antibody (ab5694)This image is courtesy of an anonymous Abreviewab5694 staining human fetal heart cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
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Western blot - alpha smooth muscle Actin antibody (ab5694)All lanes : Anti-alpha smooth muscle Actin antibody (ab5694) at 1/1000 dilution
Lane 1 : Lystates prepared from pig hear tissue from normal control animals
Lane 2 : Lystates prepared from pig hear tissue from normal control animals
Lane 3 : Lystates prepared from pig hear tissue from experimental animals
Lane 4 : Lystates prepared from pig hear tissue from experimental animals
Lysates/proteins at 4 µg per lane.
Secondary
HRP-conjugated goat polyclonal to rabbit IgG at 1/20000 dilution
Performed under reducing conditions.
Predicted band size : 42 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minuteThis image is a courtesy of Mario Torrado
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - alpha smooth muscle Actin antibody (ab5694)This image is courtesy of an Anonymous Abreviewab5694 staining alpha smooth muscle Actin in human skin tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval in Citrate pH 6.0 and then blocked with 10% serum for 1 hour at RT. The primary antibody was diluted 1/300 and incubated with sample in 2% serum for 15 hours at 4°C. A Biotin conjugated goat polyclonal to rabbit IgG was used at dilution at 1/500 as secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab5694)Image courtesy of an anonymous Abreview.ab5694 staining alpha smooth muscle Actin in rat lung tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then permeabilized using 0.5 Triton X-100 for 20 minutes, blocked with 1% BSA for 30 minutes at 20°C and then incubated with ab5694 at a 1/100 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 568 conjugated goat anti-rabbit polyclonal used at a 1/250 dilution.
References for Anti-alpha smooth muscle Actin antibody (ab5694)
This product has been referenced in:
- Khan OM et al. Targeting GGTase-I activates RHOA, increases macrophage reverse cholesterol transport, and reduces atherosclerosis in mice. Circulation 127:782-90 (2013). Mouse . Read more (PubMed: 23334894) »
- Moles A et al. Cathepsin B overexpression due to Acid sphingomyelinase ablation promotes liver fibrosis in niemann-pick disease. J Biol Chem 287:1178-88 (2012). IHC-FoFr ; Mouse . Read more (PubMed: 22102288) »
