Anti-ARID1B antibody (ab57461)

Overview

• Product nameAnti-ARID1B antibodySee all ARID1B primary antibodies ...
• Description
  Mouse monoclonal to ARID1B
• Tested applicationsWB, IHC-P, Flow Cyt more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant fragment: PPAKRHEGDM YNMQYSSQQQ EMYNQYGGSY SGPDRRPIQG QYPYPYSRER MQGPGQIQTH GIPPQMMGGP LQSSSSEGPQ QNMWAARNDM PYPYQNR, corresponding to amino acids 1364-1461 of Human ARID1B

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Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  PBS, pH 7.2
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• IsotypeIgG2b
• Light chain typekappa
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Chromatin Remodeling
  • Swi / Snf

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

Applications

Target

• RelevanceARID1B (AT-rich interactive domain-containing protein 1B) is a component of SWI/SNF chromatin remodeling complexes involved in transcriptional activation and repression of select genes by chromatin remodeling. It binds DNA non-specifically and alters DNA-nucleosome topology.
• Cellular localizationNuclear
• Database links
  • Entrez Gene: 57492 Human
  • Entrez Gene: 57492 Human
  • SwissProt: Q8NFD5 Human
  • SwissProt: Q8NFD5 Human
  • Unigene: 603283 Human

• Alternative names
  6A3 5 antibodyARID 1B antibodyARID domain containing protein 1B antibody

  AT rich interactive domain 1B (SWI1 like) antibodyAT rich interactive domain 1B antibodyAT rich interactive domain containing protein 1B antibodyBAF 250b antibodyBAF250b antibodyBRG1 associated factor 250b antibodyBRG1 binding protein antibodyBRG1 binding protein ELD/OSA1 antibodyBRG1 binding protein hELD/OSA1 antibodyBRIGHT antibodyDAN 15 antibodyDAN15 antibodyEld (eyelid)/Osa protein antibodyELD/OSA1 antibodyhELD/OSA1 antibodyhOsa 2 antibodyhOsa2 antibodyKIAA1235 antibodyOSA 2 antibodyOsa homolog 2 antibodyOSA2 antibodyOTTHUMP00000040115 antibodyp250R antibodyRP11 419L10.1 antibody

  see all

Anti-ARID1B antibody images

• 

  IHC-P - ARID1B antibody (ab57461)
  ARID1B antibody (ab57461) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human kidney.
• 

  Western blot - ARID1B antibody (ab57461)
  
  
  Predicted band size : 236 kDa
  ARID1B antibody (ab57461) at 1ug/lane + HeLa cell lysate at 25ug/lane.
• 

  Flow Cytometry-Anti-ARID1B antibody(ab57461)
  Overlay histogram showing HEK293 cells stained with ab57461 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57461, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.