Rabbit F(ab')2 polyclonal Secondary Antibody to Mouse IgG - H&L (FITC), pre-adsorbed (ab5758)

Overview

• Product nameRabbit F(ab')2 polyclonal Secondary Antibody to Mouse IgG - H&L (FITC), pre-adsorbedSee all IgG secondary antibodies ...
• Target speciesMouse
• SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.
• Tested applicationsICC/IF, Immunomicroscopy, Flow Cyt, ELISA more details
• Minimal
  cross-reactivity
  Human
  To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
  more details
• Immunogen

  Mouse IgG whole molecule

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.01% Sodium Azide
  Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
• Concentration information loading...
• Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
• Conjugation notesFluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.6 moles FITC per mole of Rabbit IgG F(ab')2
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Secondary antibodies
  • anti-Mouse
  • IgG
  • Fluorophore
  • FITC

  • Immunology
  • Immunoglobulins
  • Heavy Chain
  • IgG

Applications