Anti-Mitofusin 1 antibody (ab57602)

Overview

• Product nameAnti-Mitofusin 1 antibodySee all Mitofusin 1 primary antibodies ...
• Description
  Mouse monoclonal to Mitofusin 1
• Tested applicationsWB, IHC-P, ICC/IF, Flow Cyt, IP more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Recombinant full length protein, corresponding to amino acids 1-742 of Human Mitofusin 1

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  PBS, pH 7.2
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• IsotypeIgG2a
• Light chain typekappa
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial markers

  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitophagy fission and fusion

  • Signal Transduction
  • Second Messenger
  • Nucleotide Messenger
  • GTP

  • Tags & Cell Markers
  • Subcellular Markers
  • Organelles
  • Mitochondria

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Parkinson's disease
  • Other

Applications

Target

• FunctionEssential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks.
• Tissue specificityUbiquitous. Expressed at slightly higher level in kidney and heart. Isoform 2 may be overexpressed in some tumors, such as lung cancers.
• Sequence similaritiesBelongs to the mitofusin family.
• Post-translational
  modificationsUbiquitinated by MARCH5.
• Cellular localizationCytoplasm and Mitochondrion outer membrane.

Target information above from: UniProt accession Q8IWA4 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 55669 Human
  • Entrez Gene: 55669 Human
  • Entrez Gene: 67414 Mouse
  • Entrez Gene: 192647 Rat
  • Omim: 608506 Human
  • SwissProt: Q8IWA4 Human
  • SwissProt: Q8IWA4 Human
  • SwissProt: Q811U4 Mouse

  • SwissProt: Q8R4Z9 Rat
  • Unigene: 478383 Human
  • Unigene: 290414 Mouse
  • Unigene: 160939 Rat

  see all

• Alternative names
  Fzo homolog antibodyHfzo1 antibodyHfzo2 antibody

  MFN 1 antibodyMfn1 antibodyMFN1_HUMAN antibodyMitochondrial transmembrane GTPase Fzo 1 antibodyMitochondrial transmembrane GTPase FZO 2 antibodyMitochondrial transmembrane GTPase FZO1B antibodyMitofusin 1 antibodyMitofusin-1 antibodyMitofusin1 antibodyPutative transmembrane GTPase antibodyTransmembrane GTPase MFN1 antibody

  see all

Anti-Mitofusin 1 antibody images

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Mitofusin 1 antibody (ab57602)This image is courtesy of an anonymous Abreview

  ab57602 staining Mitofusin 1 in the H9C2 cell line from Rat cardiomyocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 1% and blocked with 5% serum for 60 minutes at 21°C. Samples were incubated with primary antibody (1/200 in PBS + 5% Goat serum) for 2 hours at 21°C. An Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal(1/400) was used as the secondary antibody.

  See Abreview

• 

  Western blot - Mitofusin 1 antibody (ab57602)
  
  
  Predicted band size : 84 kDa
  Mitofusin 1 antibody (ab57602) at 1ug/lane + HeLa cell lysate at 25ug/lane.
• 

  Immunocytochemistry/ Immunofluorescence-Mitofusin 1 antibody(ab57602)
  ICC/IF image of ab57602 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57602, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Mitofusin 1 antibody(ab57602)
  IHC image of ab57602 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab57602, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  
  
• 

  Flow Cytometry-Mitofusin 1 antibody(ab57602)
  Overlay histogram showing HEK293 cells stained with ab57602 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57602, 1µg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.
  
  
• 

  Immunoprecipitation - Anti-Mitofusin 1 antibody (ab57602)
  Mitofusin 1 was immunoprecipitated using 0.5mg Hela whole cell extract, 10ug of Mouse monoclonal to Mitofusin 1 and 50µl of protein G magnetic beads (lane 1). The antibody was incubated with the Protein G beads for 10 min under agitation. No antibody was added to the control lane 2 and no extract or antibody was added to contol lane 3. Hela whole cell extract diluted in RIPA buffer was added to each sample and incubated for 10min under agitation. Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70^oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab57602.
  Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
  Bands: 84kDa:Mitofusin 1; 60kDa bead background: non specific - 48kDa: We are unsure as to the identity of this extra band.