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Overview

  • Product nameAnti-Dub3 antibodySee all Dub3 primary antibodies ...
  • Description
    Mouse monoclonal to Dub3
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment: ESTLDHWKFL QEQNKTKPEF NVRKVEGTLP PDVLVIHQSK YKCGMKNHHP EQQSSLLNLS STTPTHQESM NTGTLASLRG RARRSKGKNK HSKRALLVCQ , corresponding to amino acids 431-531 of Human Dub3

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

Applications

Our Abpromise guarantee covers the use of ab57801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionDeubiquitinating enzyme that specifically removes conjugated ubiquitin from CDC25A, thereby playing a key role in cell cycle regulation. Deubiquitination of CDC25A prevents its degradation by the proteasome during the G1/S and G2/M phases of the cell cycle and is required for cell-cycle progression. Has transforming capabilities on cell lines. May also function in cell apoptosis.
    • Tissue specificityBroadly expressed.
    • Sequence similaritiesBelongs to the peptidase C19 family. USP17 subfamily.
    • Cellular localizationNucleus.

      • Target information above from: UniProt accession Q6R6M4 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          Cytokine inducible Deubiquitinating Enzyme antibodyDeubiquitinating enzyme 17-like protein 2 antibodyDeubiquitinating enzyme 3 antibody
          Deubiquitinating protein 3 antibodyDUB-3 antibodyDUB3 protein antibodyU17L2_HUMAN antibodyUbiquitin carboxyl-terminal hydrolase 17-like protein 2 antibodyUbiquitin thiolesterase 17-like protein 2 antibodyUbiquitin-specific-processing protease 17-like protein 2 antibodyUSP17L2 antibody
        see all

      Anti-Dub3 antibody images

      • Western blot - Dub3 antibody (ab57801)
        Western blot - Dub3 antibody (ab57801)
        Western blot against tagged recombinant protein immunogen using ab57801 Dub3 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

      References for Anti-Dub3 antibody (ab57801)

      ab57801 has not yet been referenced specifically in any publications.

      Publishing research using ab57801 ? Please let us know so that we can cite the reference in this datasheet

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      Western blot abreview for Anti-Dub3 antibody

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      Abreviews
      Application Western blot
      Sample Human Cell lysate - whole cell (breast and colon cancer cell)
      Loading amount 100 µg
      Specification breast and colon cancer cell
      Gel Running Conditions Non-reduced Denaturing (10% SDS_PAGE)
      Blocking step Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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      Submitted May 22 2012

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"