Overview
- Product nameAnti-StAR antibodySee all StAR primary antibodies ...
- DescriptionMouse monoclonal to StAR
- Tested applicationsWB, ELISA, IHC-Fr more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
Recombinant fragment: EAMQKALGIL SNQEGWKKES QQDNGDKVMS KVVPDVGKVF RLEVVVDQPM ERLYEELVER MEAMGEWNPN VKEIKVLQKI GKDTFITHEL AAEAAGNLVG , corresponding to amino acids 81-180 of Human StAR
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
PBS, pH 7.2 -
Concentration information loading... - PurityProtein G purified
- Clonality Monoclonal
- IsotypeIgG2a
- Light chain typekappa
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab58013 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 - 5 µg/ml. |
| ELISA | ELISA: Use at an assay dependent dilution. |
| IHC-Fr | IHC-Fr: Use at an assay dependent dilution. PubMed: 21094146 |
Target
- FunctionPlays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone.
- Tissue specificityExpressed in gonads, adrenal cortex and kidney.
- PathwaySteroid metabolism; cholesterol metabolism.
- Involvement in diseaseDefects in STAR are the cause of adrenal hyperplasia type 1 (AH1) [MIM:201710]. The most severe form of adrenal hyperplasia. It is a condition characterized by onset of profound adrenocortical insufficiency shortly after birth, hyperpigmentation reflecting increased production of pro-opiomelanocortin, elevated plasma renin activity as a consequence of reduced aldosterone synthesis, and male pseudohermaphroditism resulting from deficient fetal testicular testosterone synthesis. Affected individuals are phenotypic females irrespective of gonadal sex, and frequently die in infancy if mineralocorticoid and glucocorticoid replacement are not instituted.
- Sequence similaritiesContains 1 START domain.
- Cellular localizationMitochondrion.
-
Database links
- Entrez Gene: 6770 Human
- Entrez Gene: 20845 Mouse
- Entrez Gene: 25557 Rat
- Omim: 600617 Human
- SwissProt: Q6IBK0 Human
- SwissProt: P49675 Human
- SwissProt: P51557 Mouse
- SwissProt: P97826 Rat
- Unigene: 521535 Human
- Unigene: 293314 Mouse
- Unigene: 11399 Rat
see all
Target information above from: UniProt accession
P49675
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
-
Alternative names
- Cholesterol trafficker antibodyLuteinizing hormone induced protein antibodymitochondrial antibody
- Mitochondrial steroid acute regulatory protein antibodyStAR antibodyStAR related lipid transfer (START) domain containing 1 antibodySTAR_HUMAN antibodyStARD1 antibodySTART domain containing 1 antibodySTART domain containing protein 1 antibodySTART domain-containing protein 1 antibodySteroid acute regulatory protein antibodySteroidogenic acute regulator antibodySteroidogenic acute regulatory protein antibodySteroidogenic acute regulatory protein mitochondrial antibody
see all
Anti-StAR antibody images
-
Western blot - StAR antibody (ab58013)Anti-StAR antibody (ab58013) at 1/1000 dilution + Mouse liver lysates at 50 µg
Secondary
HRP-conjugated Goat polyclonal to mouse IgG at 1/2500 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 27,30 kDa (why is the actual band size different from the predicted?)
Exposure time : 5 minutes -
Western blot - StAR antibody (ab58013)Anti-StAR antibody (ab58013) at 1/1000 dilution (in TBST + 2.5% milk for 16 hours at 4°C) + Whole cell lysate of human THP1 cells at 50 µg
Secondary
An HRP-conjugated Goat anti-mouse IgG polyclonal at 1/2000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
Exposure time : 2 minutesThis image is courtesy of an anonymous Abreview
References for Anti-StAR antibody (ab58013)
This product has been referenced in:
- Hannen RF et al. Steroid synthesis by primary human keratinocytes; implications for skin disease. Biochem Biophys Res Commun 404:62-7 (2011). IHC-Fr ; Human . Read more (PubMed: 21094146) »
- Ramanjaneya M et al. Orexins stimulate steroidogenic acute regulatory protein expression through multiple signaling pathways in human adrenal H295R cells. Endocrinology 149:4106-15 (2008). WB ; Human . Read more (PubMed: 18450961) »
