Overview
- Product nameAnti-G protein alpha antibodySee all G protein alpha primary antibodies ...
- DescriptionRabbit polyclonal to G protein alpha
- Tested applicationsWB, IHC-P more details
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig - Immunogen
A region within synthetic peptide: YRATHRLLLL GAGESGKSTI VKQMRILHVN GFNGDSEKAT KVQDIKNNLK, corresponding to N terminal amino acids 37-86 of Human G protein alpha
- Positive controlWB: HepG2 membrane lysate IHC-P: Human kidney tissue and human lung tissue
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... - PurityProtein A purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab58916 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 2.5 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 46 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
| IHC-P | IHC-P: Use at an assay dependent concentration. |
Target
- RelevanceGTP-binding proteins (G-proteins)are a family of heterotrimeric proteins that play a critical role in signal transduction by coupling cell surface, 7-transmembrane domain receptors to intracellular signaling pathways including second messenger generation (such as cyclic AMP, calcium and diacylglycerol), protein phosphorylation, ion channel activation, gene induction, cell growth and differentiation. Receptor activation catalyzes the exchange of GTP for GDP bound to the inactive G protein alpha subunit resulting in a conformational change and dissociation of the complex. The G protein alpha and beta-gamma subunits are capable of regulating various cellular effectors. Activation is terminated by a GTPase intrinsic to the G-alpha subunit.
- Cellular localizationCell Membrane
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Database links
- Entrez Gene: 281793 Cow
- Entrez Gene: 2778 Human
- Entrez Gene: 14683 Mouse
- Entrez Gene: 100049657 Pig
- Entrez Gene: 24896 Rat
- Omim: 139320 Human
- SwissProt: Q5JWF2 Human
- SwissProt: P63092 Human
- SwissProt: P63094 Mouse
- SwissProt: Q6R0H7 Mouse
- SwissProt: P29797 Pig
- SwissProt: P63095 Rat
- SwissProt: Q63803 Rat
- Unigene: 125898 Human
- Unigene: 125770 Mouse
- Unigene: 31 Rat
see all
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Alternative names
- Adenylate cyclase stimulating G alpha protein antibodyAHO antibodyC20orf45 antibody
- Extra large alphas protein antibodyGNAS complex locus antibodyGNAS1 antibodyGPSA antibodyGSA antibodyGSP antibodyGuanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 antibodyGuanine nucleotide binding protein G(s) alpha subunit antibodyGuanine nucleotide binding protein G(s) subunit alpha isoforms XLas antibodyguanine nucleotide regulatory protein antibodyNESP antibodyNeuroendocrine secretory protein antibodyPHP1A antibodyPHP1B antibodyPHP1C antibodyPOH antibodyRP4-543J19.4 antibodySecretogranin VI antibodyXLalphas antibody
see all
Anti-G protein alpha antibody images
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Western blot - G protein alpha antibody (ab58916)Anti-G protein alpha antibody (ab58916) at 2.5 µg/ml + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 46 kDa
Observed band size : 43 kDa (why is the actual band size different from the predicted?) -
Immunohistochemistry (Paraffin-embedded sections) - G protein alpha antibody (ab58916)This image shows human kidney tissue stained with ab58916 at 8µg/ml. The arrows point to epithelial cells of the renal tubule (positive label); X400 magnification. -
Immunohistochemistry (Paraffin-embedded sections) - G protein alpha antibody (ab58916)This image shows human lung tissue stained with ab58916 at 8µg/ml. The arrows point to alveolar cells (positive label); X400 magnification. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-G protein alpha antibody(ab58916)Immunohistochemistry of Human Lung lysate tissue at an antibody concentration of 4-8µg/ml using anti-GNAS antibody (ab58916) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-G protein alpha antibody(ab58916)Immunohistochemistry of Human kidney lysate tissue at an antibody concentration of 4-8µg/ml using anti-GNAS antibody (ab58916)
References for Anti-G protein alpha antibody (ab58916)
ab58916 has not yet been referenced specifically in any publications.
