Anti-CRF1 antibody (ab59023)

Overview

• Product nameAnti-CRF1 antibodySee all CRF1 primary antibodies ...
• Description
  Goat polyclonal to CRF1
• Tested applicationsIHC-P, ELISA, IHC-FoFr, WB more details
• Species reactivity
  Reacts with: Mouse
  Predicted to work with: Rat, Sheep, Chicken, Human, Pufferfish (Fugu), Rhesus monkey, African Green Monkey, Syrian Hamster
• Immunogen

  Synthetic peptide: KLYYDNEKCWFGKR by a Cysteine residue linker, corresponding to internal sequence amino acids 579-292 of Human CRF1(Peptide available as ab113546.)

  Run BLAST with Run BLAST with

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Obesity

  • Metabolism
  • Types of disease
  • Cancer

  • Signal Transduction
  • Signaling Pathway
  • Nuclear Signaling
  • Nuclear Hormone Receptors
  • Corticoid

  • Neuroscience
  • Endocrine system
  • Hypothalamic pituitary adrenal axis

  • Neuroscience
  • Neurology process
  • Metabolism

Applications

Target

• FunctionThis is a receptor for corticotropin releasing factor. Shows high-affinity CRF binding. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
• Tissue specificityPredominantly expressed in the cerebellum, pituitary, cerebral cortex and olfactory lobe.
• Sequence similaritiesBelongs to the G-protein coupled receptor 2 family.
• Post-translational
  modificationsC-terminal Ser or Thr residues may be phosphorylated.
• Cellular localizationCell membrane.

Target information above from: UniProt accession P34998 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1394 Human
  • Entrez Gene: 12921 Mouse
  • Entrez Gene: 58959 Rat
  • Omim: 122561 Human
  • SwissProt: P34998 Human
  • SwissProt: P35347 Mouse
  • SwissProt: P35353 Rat
  • Unigene: 417628 Human

  • Unigene: 1892 Mouse
  • Unigene: 10499 Rat

  see all

• Alternative names
  Corticotropin releasing factor type 1 receptor antibodycorticotropin releasing hormone receptor 1 antibodyCorticotropin-releasing factor receptor 1 antibody

  Corticotropin-releasing hormone receptor 1 antibodyCRF R antibodycrf receptor 1 antibodycrf receptor type 1 antibodycrf type 1 antibodyCRF-R antibodyCRF-R-1 antibodyCRF-R1 antibodyCRF1 antibodyCRFR antibodyCRFR-1 antibodyCRFR1 antibodyCRFR1_HUMAN antibodyCRH-R-1 antibodyCRH-R1 antibodyCRH-R1h antibodyCRHR antibodyCrhr1 antibodyCRHR1f antibodyCRHR1L antibody

  see all

Anti-CRF1 antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CRF1 antibody (ab59023)This image is courtesy of an Abreview submitted by Dr Christoph Schwarzer
  ab59023 (1/100) staining CRF1 in paraffin embedded mouse brain tissue sections. Tissues were fixed in paraformaldehyde and permeabilised with 0.3% Triton X100/TBS. Please refer to abreview for further experimental details.

  See Abreview

• 

  Western blot - CRF1 antibody (ab59023)
  All lanes : Anti-CRF1 antibody (ab59023) at 1 µg/ml
  
  Lane 1 : Rat Forebrain
  Lane 2 : Rat Cortex Tissue Lysate
  Lane 3 : Cerebellum Rat Tissue Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 50 kDa
  Observed band size : 50 kDa
  Additional bands at : 110 kDa,70 kDa,75 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 8 minutes