Anti-Anillin antibody (ab5910)

Overview

• Product nameAnti-Anillin antibodySee all Anillin primary antibodies ...
• Description
  Goat polyclonal to Anillin
• Tested applicationsICC, IHC-P more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide: WQPDACYKPIGKP, corresponding to C terminal amino acids 1113-1125 of Human Anillin.

  Run BLAST with Run BLAST with
• General notesGenBank Accession Number – NP_061155

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 0.5% BSA, Tris-saline. pH 7.3
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microfilaments
  • Actin etc
  • Actin Binding Proteins

  • Cell Biology
  • Cell Cycle
  • Cell Division
  • Cytokinesis

Applications

Target

• FunctionRequired for cytokinesis. Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression.
• Tissue specificityUbiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors.
• Sequence similaritiesContains 1 PH domain.
• Developmental stageExpressed in fetal brain, heart, kidney, liver, lung, skeletal muscle, spleen and thymus. In dividing cells expression increases during S and G2 phases, peaks at mitosis and subsequently drops as cells enter G1 phase.
• Post-translational
  modificationsPhosphorylated during mitosis.
  Ubiquitinated, and this requires FZR1/CDH1.
• Cellular localizationNucleus. Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Mainly found in the nucleus during interphase. Colocalizes with cortical F-actin upon nuclear envelope breakdown in mitosis and subsequently concentrates in the area of the prospective contractile ring in anaphase. This pattern persists until telophase, when the protein becomes concentrated in the midbody.

Target information above from: UniProt accession Q9NQW6 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 54443 Human
  • SwissProt: Q9NQW6 Human
  • Unigene: 62180 Human

• Alternative names
  Actin binding protein scraps homolog Drosophila antibodyActin binding protein anillin antibodyActin-binding protein anillin antibody

  Anillin actin binding protein antibodyANILLIN antibodyANLN antibodyANLN_HUMAN antibodyDKFZp779A055 antibodyScra antibodyScraps antibodyScraps homolog antibody

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Anti-Anillin antibody images

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Anillin antibody (ab5910)This image is courtesy of an Abreview submitted by Manoj B Menon

  ab14404 staining Anillinin Mouse fibroblast cellsby ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with methanol and blocked with 4% BSA for 2 hours at 4°C. Samples were incubated with primary antibody (1/25 in PBS + 1% BSA) for 2 hour at 25°C. An  Alexa Fluor®488-conjugated Donkey anti-goat  polyclonal(1/500) was used as the secondary antibody.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anillin antibody (ab5910)

  ab5910 at 10ug/ml staining Anillin in human kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in microwave and in Tris/EDTA buffer (pH 9.0). HRP-staining procedure was used for detection.