Anti-Anillin antibody (ab5910)
- Product nameAnti-Anillin antibodySee all Anillin primary antibodies ...
- DescriptionGoat polyclonal to Anillin
- Tested applicationsICC, IHC-P more details
- Species reactivityReacts with: Human
Synthetic peptide: WQPDACYKPIGKP, corresponding to C terminal amino acids 1113-1125 of Human Anillin.
- General notesGenBank Accession Number – NP_061155
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3
- Concentration information loading...
- PurityImmunogen affinity purified
- Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab5910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||ICC: Use at an assay dependent concentration.|
|IHC-P||IHC-P: Use a concentration of 3 - 10 µg/ml.|
- FunctionRequired for cytokinesis. Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression.
- Tissue specificityUbiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors.
- Sequence similaritiesContains 1 PH domain.
- Developmental stageExpressed in fetal brain, heart, kidney, liver, lung, skeletal muscle, spleen and thymus. In dividing cells expression increases during S and G2 phases, peaks at mitosis and subsequently drops as cells enter G1 phase.
modificationsPhosphorylated during mitosis.
Ubiquitinated, and this requires FZR1/CDH1.
- Cellular localizationNucleus. Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Mainly found in the nucleus during interphase. Colocalizes with cortical F-actin upon nuclear envelope breakdown in mitosis and subsequently concentrates in the area of the prospective contractile ring in anaphase. This pattern persists until telophase, when the protein becomes concentrated in the midbody.
- Actin binding protein scraps homolog Drosophila antibodyActin binding protein anillin antibodyActin-binding protein anillin antibody
- Anillin actin binding protein antibodyANILLIN antibodyANLN antibodyANLN_HUMAN antibodyDKFZp779A055 antibodyScra antibodyScraps antibodyScraps homolog antibody
Anti-Anillin antibody images
ab14404 staining Anillinin Mouse fibroblast cellsby ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with methanol and blocked with 4% BSA for 2 hours at 4°C. Samples were incubated with primary antibody (1/25 in PBS + 1% BSA) for 2 hour at 25°C. An Alexa Fluor®488-conjugated Donkey anti-goat polyclonal(1/500) was used as the secondary antibody.
ab5910 at 10ug/ml staining Anillin in human kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in microwave and in Tris/EDTA buffer (pH 9.0). HRP-staining procedure was used for detection.
References for Anti-Anillin antibody (ab5910)
This product has been referenced in:
- Stoeckelhuber M et al. Human axillary apocrine glands: proteins involved in the apocrine secretory mechanism. Histol Histopathol 26:177-84 (2011). IHC-P ; Human . Read more (PubMed: 21154231) »
- Hall PA et al. The septin-binding protein anillin is overexpressed in diverse human tumors. Clin Cancer Res 11:6780-6 (2005). IHC-P ; Human . Read more (PubMed: 16203764) »