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Overview

  • Product nameAnti-intestinal FABP antibodySee all intestinal FABP primary antibodies ...
  • Description
    Goat polyclonal to intestinal FABP
  • Tested applicationsELISA, WB, IHC-P, ICC/IF more details
  • Species reactivity
    Reacts with: Cat, Human
    Predicted to work with: Rat, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide: C-EGVEAKRIFKKD, corresponding to C terminal amino acids 121-132 of Human intestinal FABP

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive controlHuman Duodenum lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab60272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1:64000.
WB WB: Use a concentration of 0.001 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 15 kDa).Can be blocked with intestinal FABP peptide (ab115238).
IHC-P IHC-P: Use a concentration of 2 - 3 µg/ml.
ICC/IF ICC/IF: Use at an assay dependent concentration. PubMed: 23236492

Target

  • FunctionFABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.
  • Tissue specificityExpressed in the small intestine and at much lower levels in the large intestine. Highest expression levels in the jejunum.
  • Sequence similaritiesBelongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • DomainForms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.
  • Cellular localizationCytoplasm.

    • Target information above from: UniProt accession P12104 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      FABP 2 antibodyFABP2 antibodyFABPI antibody
      FABPI_HUMAN antibodyFatty acid binding protein 2 intestinal antibodyFatty acid binding protein antibodyFatty acid binding protein intestinal antibodyFatty acid-binding protein 2 antibodyFatty acid-binding protein antibodyI FABP antibodyI-FABP antibodyIFABP antibodyintestinal antibodyIntestinal fatty acid binding protein 2 antibodyIntestinal-type fatty acid-binding protein antibodyMGC133132 antibodyOTTHUMP00000163925 antibody
    see all

Anti-intestinal FABP antibody images

  • Western blot - intestinal FABP antibody (ab60272)
    Western blot - intestinal FABP antibody (ab60272)
    ab60272 antibody (0.001µg/ml) staining of intestinal FABP in Human Duodenum lysate (35µg protein in RIPA buffer)

    Predicted band size : 15 kDa
    Observed band size : 16 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - intestinal FABP antibody (ab60272)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - intestinal FABP antibody (ab60272)

    ab60272 staining FABP in Human small intestine by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The tissue sections underwent antigen retrieval by steam with citrate buffer pH 6.0. The AP staining procedure was used for detection.

References for Anti-intestinal FABP antibody (ab60272)

This product has been referenced in:
  • Treutlein G  et al. Novel potential interacting partners of fibronectin in spontaneous animal model of interstitial cystitis. PLoS One 7:e51391 (2012). WB, ICC/IF ; Cat . Read more (PubMed: 23236492) »

See 1 Publication for this product

Publishing research using ab60272 ? Please let us know so that we can cite the reference in this datasheet

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Customer called to ask about the appropriate secondary antibody for ab60272 and inquire about the 100% Abreview testing discount.

I am very pleased to hear you would like to accept our offer and test ab60272 in ELISA on Rhesus Monkey. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for ELISA on Rhesus Monkey...

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Interested in blocking peptide.

Thank you for your calls last week and for your patience while I have worked to have this peptide added to our catalog. The peptide is now available as ab115238- http://www.abcam.com/intestinal-FABP-peptide-ab115238.html Please let me know if the...

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