Overview
Product namePI 3 Kinase p110 delta + PI 3 Kinase p85 alpha protein (Active)
Protein descriptionRecombinant full length PI 3 Kinase p110 delta (tagged) and PI 3 Kinase p85 alpha proteins (Human), co-expressed in a Baculovirus infected Sf9 cell expression system, MWs 146 and 83.5 kDa respectively.
Expression hostBaculovirus infected sf9 cells
Properties
Purification notesPurity: >80% as determined by SDS-PAGE.
FormLiquid
Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
Storage bufferPreservative: None
Constituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8
Concentration information loading...
Additional notesFull length p110 Delta - Tagged.
Full length p85 - NOT TAGGED.
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab60892
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
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Notes |
| SDS-PAGE |
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Application notesSDS-PAGE: Use at an assay dependent dilution.
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Specific activity: 3 nmole/mg/min using phosphatidylinositol as the substrate.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
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Alternative names
p85 ALPHAPhosphoinositide 3 kinase CPI3 kinase p110 subunit deltaPI3 kinase p85 subunit alphaPI3KPIK3CDPIK3R1PtdIns 3 kinase p110PtdIns 3 kinase p85 alpha
see all
RelevancePI3-Kinases (PI3-Ks) are a family of lipid kinases that are implicated in signal transduction. PI3-K consists of two subunits; p85 and p110. The p85 subunit localize PI3-K activity to the plasma membrane while the p110 subunit contains the catalytic domain of PI3-K. Four isoforms of p110 has been found; the alpha, beta, gamma, and the delta subunit. The delta isoform is predominantly expressed in leukocytes and has been shown to interact with p85 and GTP-bound Ras via its SH2/SH3 domain.
The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that is thought to phosphorylate second messengers in growth signalling pathways. PI3K exists as a heterodimer of catalytic 110 kDa (p110) and regulatory 85 kDa (p85, p50 or p55) subunits. The p85 unit consists of two closely related proteins: p85 alpha, which binds tightly to the catalytic subunit and p85 beta, a protein of unknown function. They share 62% amino acid sequence identity. p85 alpha lacks PI3 kinase activity, but may serve as regulator of the catalytic subunit, p110, by acting as the link between PI3K and the ligand activated receptor. Both isoforms bind to activated receptors and serve as tyrosine kinase substrates.
Cellular localizationCytoplasmic
PI 3 Kinase p110 delta + PI 3 Kinase p85 alpha protein (Active) images
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SDS-PAGE - PI 3 Kinase p110 delta + PI 3 Kinase p85 alpha protein (ab60892)
Lane 1: 3µg of ab60892.Lane 2: Protein marker.
References for PI 3 Kinase p110 delta + PI 3 Kinase p85 alpha protein (Active) (ab60892)
ab60892
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
