Anti-ADAM17 (phospho T735) antibody (ab60996)
Overview
- Product nameAnti-ADAM17 (phospho T735) antibodySee all ADAM17 primary antibodies ...
- DescriptionRabbit polyclonal to ADAM17 (phospho T735)
- SpecificityDetects ADAM 17 only when phosphorylated at threonine 735.
- Tested applicationsELISA, WB more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
Synthetic phosphopeptide derived from human ADAM 17 around the phosphorylation site of threonine 735 (P-Q-TP-P-G).
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesThe antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
- Clonality Polyclonal
- IsotypeIgG
-
Research Areas
Applications
Our Abpromise guarantee covers the use of ab60996 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | |
| WB |
WB: 1/500 - 1/1000. Detects a band of approximately 93 kDa (predicted molecular weight: 93 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- FunctionCleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Also involved in the activation of Notch pathway.
- Tissue specificityUbiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.
- Sequence similaritiesContains 1 disintegrin domain.
Contains 1 peptidase M12B domain. - DomainMust be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Only the catalytic domain is essential to shed TNF and p75 TNFR.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. - Post-translational
modificationsThe precursor is cleaved by a furin endopeptidase.
Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791. - Cellular localizationMembrane.
-
Database links
- Entrez Gene: 6868 Human
- Entrez Gene: 11491 Mouse
- Entrez Gene: 57027 Rat
- Omim: 603639 Human
- SwissProt: P78536 Human
- SwissProt: Q9Z0F8 Mouse
- SwissProt: Q9Z1K9 Rat
- Unigene: 404914 Human
- Unigene: 27681 Mouse
- Unigene: 144585 Rat
see all
Target information above from: UniProt accession
P78536
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
-
Alternative names
- A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme) antibodyA disintegrin and metalloproteinase domain 17 antibodyADA17_HUMAN antibody
- ADAM 17 antibodyADAM metallopeptidase domain 17 antibodyADAM17 antibodyADAM17 protein antibodyCD 156b antibodyCD156b antibodyCD156b antigen antibodyCSVP antibodyDisintegrin and metalloproteinase domain-containing protein 17 antibodyMGC71942 antibodySnake venom like protease antibodySnake venom-like protease antibodyTACE antibodyTNF alpha convertase antibodyTNF alpha converting enzyme antibodyTNF-alpha convertase antibodyTNF-alpha-converting enzyme antibodyTumor Necrosis Factor Alpha Converting Enzyme antibody
see all
Anti-ADAM17 (phospho T735) antibody images
-
Western blot - ADAM17 (phospho T735) antibody (ab60996)All lanes : Anti-ADAM17 (phospho T735) antibody (ab60996) at 1/500 dilution
Lane 1 : Extracts from K562 cells, treated with UV (5mins).
Lane 2 : Extracts from K562 cells, treated with UV (5mins) with immunizing phosphopeptide.
Predicted band size : 93 kDa
Observed band size : 90 kDa (why is the actual band size different from the predicted?) -
- ADAM17 (phospho T735) antibody (ab60996)
References for Anti-ADAM17 (phospho T735) antibody (ab60996)
This product has been referenced in:
- Thorp E et al. Shedding of the Mer Tyrosine Kinase Receptor Is Mediated by ADAM17 Protein through a Pathway Involving Reactive Oxygen Species, Protein Kinase C{delta}, and p38 Mitogen-activated Protein Kinase (MAPK). J Biol Chem 286:33335-44 (2011). Read more (PubMed: 21828049) »
- Hilliard VC et al. TNF-a converting enzyme-mediated ErbB4 transactivation by TNF promotes colonic epithelial cell survival. Am J Physiol Gastrointest Liver Physiol 301:G338-46 (2011). WB ; Mouse . Read more (PubMed: 21617117) »
