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Overview

  • Product nameAnti-Histone H2B antibodySee all Histone H2B primary antibodies ...
  • Description
    Rabbit polyclonal to Histone H2B
  • Tested applicationsELISA, ICC/IF, IHC-P, WB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic non-acetylated peptide derived from human Histone H2B (Swiss-Prot P57053) from around the acetylation site of lysine 12 (P-K-KA-G-S).

  • Positive controlHuman breast carcinoma tissue, COS7 cell extracts treated with TSA (400nM, 24hours) and HeLa cells.

Properties

  • FormLiquid
  • Storage instructionsStore at -20°C. Stable for 12 months at -20°C
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab61250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA ELISA: 1/5000.
ICC/IF ICC/IF: 1/500 - 1/1000.
IHC-P IHC-P: 1/50 - 1/100.
WB WB: 1/500 - 1/1000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).

Target

  • RelevanceIn diploid eukaryotic cells, the chromatin fibers are about 20nM in diameter. They consist of two major components in equal amounts, DNA and basic proteins called histones. Histones are believed to be regularly arranged in the deep grove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3 and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Linker histones are involved in the formation of higher order structure in chromatin and the maintenance of overall chromatin compaction. Whilst the core histones are highly conserved across a wide range of organisms, the linker histones are less conserved.
  • Cellular localizationNuclear
  • Database links
    see all
  • Alternative names
      H2B GL105 antibodyH2B histone family member O antibodyH2B histone family member S antibody
      H2B.1 antibodyH2B.1 B antibodyH2B.b antibodyH2B.c antibodyH2B.d antibodyH2B.e antibodyH2B.f antibodyH2B.j antibodyH2B.q antibodyH2B/b antibodyH2B/c antibodyH2B/d antibodyH2B/e antibodyH2B/f antibodyH2B/j antibodyH2B/o antibodyH2B/q antibodyH2BFB antibodyH2BFC antibodyH2BFD antibodyH2BFE antibodyH2BFF antibodyH2BFJ antibodyH2BFO antibodyH2BFQ antibodyH2BFS antibodyHIRIP2 antibodyHIST1H2BB antibodyHIST1H2BD antibodyHIST1H2BH antibodyHIST1H2BL antibodyHIST1H2BM antibodyHIST1H2BN antibodyHIST2H2BE antibodyHistone H2B antibodyHistone H2B type 1 B antibodyHistone H2B type 1 D antibodyHistone H2B type 1 H antibodyHistone H2B type 1 L antibodyHistone H2B type 1 M antibodyHistone H2B type 1 N antibodyHistone H2B type 2 E antibodyhistone protein antibody
    see all

Anti-Histone H2B antibody images

  • Immunocytochemistry/ Immunofluorescence - Histone H2B antibody (ab61250)
    Immunocytochemistry/ Immunofluorescence - Histone H2B antibody (ab61250)
    ab61250 at 1/500 - 1/1000 dilution staining Histone H2B in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H2B antibody (ab61250)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H2B antibody (ab61250)
    ab61250 at 1/50 - 1/100 dilution staining Histone H2B in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
  • Western blot - Histone H2B antibody (ab61250)
    Western blot - Histone H2B antibody (ab61250)
    All lanes : Anti-Histone H2B antibody (ab61250) at 1/500 dilution

    Lane 1 : COS7 cell extracts treated with TSA (400nM, 24hours)
    Lane 2 : COS7 cell extracts treated with TSA (400nM, 24hours) with the immunising peptide


    Predicted band size : 14 kDa
    Observed band size : 14 kDa

References for Anti-Histone H2B antibody (ab61250)

This product has been referenced in:
  • Colpitts TM  et al. Dengue virus capsid protein binds core histones and inhibits nucleosome formation in human liver cells. PLoS One 6:e24365 (2011). WB, ICC/IF ; Human . Read more (PubMed: 21909430) »

See 1 Publication for this product

Publishing research using ab61250 ? Please let us know so that we can cite the reference in this datasheet

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Western blot abreview for Anti-Histone H2B antibody

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Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (Human Caco-2 epithelial colorectal adenocarcinoma)
Loading amount 10 µg
Specification Human Caco-2 epithelial colorectal adenocarcinoma
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Dec 15 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"