EGFR protein (Tagged) (ab61264)
Constituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, 10mM Glutathione, pH 8.0
Our Abpromise guarantee covers the use of ab61264 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Specific Activity: 140 U/µg.
One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Tyr substrate per minute at pH 7.4 and 30°C.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- Avian erythroblastic leukemia viral (v erb b) oncogene homologCell growth inhibiting protein 40Cell proliferation inducing protein 61
- EGF REGFREGFR_HUMANEpidermal growth factor receptorEpidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog)Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian)Erbberbb 1Erbb1ERBB1ErrpHER1mENAOncogene ERBBPIG61Proto-oncogene c-ErbB-1Receptor tyrosine protein kinase ErbB 1Receptor tyrosine-protein kinase ErbB-1Urogastronewa2Wa5
Isoform 2 may act as an antagonist of EGF action.
Contains 1 protein kinase domain.
modificationsPhosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3.
Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur. Deubiquitinated by OTUD7B, preventing degradation.
Methylated. Methylation at Arg-1199 by PRMT5 positively stimulates phosphorylation at Tyr-1197.
EGFR protein (Tagged) images
References for EGFR protein (Tagged) (ab61264)
ab61264 has not yet been referenced specifically in any publications.