You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Overview

  • Product nameEGFR protein (Tagged)See all EGFR proteins and peptides ...
  • Protein descriptionRecombinant (cytoplasmic domain) fragment, corresponding to amino acids 668-1210 of Human EGFR. Swiss-Prot ID = P00533 (NM_005228 isoform a) with an N-terminal enzyme tag. MW 87.3 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
  • Expression hostInsect cells

    • Properties

  • Purification notesPurity: >30% by SDS-PAGE.
  • FormLiquid
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, 10mM Glutathione, pH 8.0
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab61264 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies
  • Application notesKinase Activity: Use at an assay dependent dilution.

    Specific Activity: 140 U/µg.

    One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Tyr substrate per minute at pH 7.4 and 30°C.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Avian erythroblastic leukemia viral (v erb b) oncogene homologCell growth inhibiting protein 40Cell proliferation inducing protein 61
        EGF REGFREGFR_HUMANEpidermal growth factor receptorEpidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog)Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian)Erbberbb 1Erbb1ERBB1ErrpHER1mENAOncogene ERBBPIG61Proto-oncogene c-ErbB-1Receptor tyrosine protein kinase ErbB 1Receptor tyrosine-protein kinase ErbB-1Urogastronewa2Wa5
      see all
  • FunctionReceptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
    Isoform 2 may act as an antagonist of EGF action.
  • Tissue specificityUbiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
  • Involvement in diseaseDefects in EGFR are associated with lung cancer (LNCR) [MIM:211980]. LNCR is a common malignancy affecting tissues of the lung. The most common form of lung cancer is non-small cell lung cancer (NSCLC) that can be divided into 3 major histologic subtypes: squamous cell carcinoma, adenocarcinoma, and large cell lung cancer. NSCLC is often diagnosed at an advanced stage and has a poor prognosis.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications    Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3.
    Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur. Deubiquitinated by OTUD7B, preventing degradation.
    Methylated. Methylation at Arg-1199 by PRMT5 positively stimulates phosphorylation at Tyr-1197.
  • Cellular localizationSecreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand.

    • Target information above from: UniProt accession P00533 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    EGFR protein (Tagged) images

    • Sandwich ELISA - EGFR protein (Tagged) (ab61264)
      Sandwich ELISA - EGFR protein (Tagged) (ab61264)
      Standard curve for EGFR (Analyte: ab61264); dilution range 1pg/ml to 1µg/ml using Capture Antibody ab62 at 5µg/ml and Detector Antibody ab2430 at 0.5µg/ml.

    References for EGFR protein (Tagged) (ab61264)

    ab61264 has not yet been referenced specifically in any publications.

    Publishing research using ab61264 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit a Question for ab61264

    Displaying 1 - 1 of 1 results for Q&A

    10
    Votes: Highest First

    Hi Karen. I need to perform this as an experimental assay to detect the EGFR through the detection of my gold nanoparticles - I don't need any fluorescent molecules. If this first step works well I will have to do the same assay to detect EGFR from ce...

    Read More

    Thank you for your reply. I'm assuming the EGFR will be taken from human cancer cells? This does sound similar to a sandwich ELISA. For sandwich ELISA, we would usually recommend using a polyclonal to pull down as much of the protein as p...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"